ISSN: 2451-0637
Archives of Medical Science - Civilization Diseases
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1/2021
vol. 6
 
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abstract:
Experimental research

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

Ameera Kamal Khaleel
1
,
Ramizu Bin Shaari
1
,
Mohamad Arif Awang Nawi
1
,
Ali Mihsen Al-Yassiri
2

1.
School of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kota Bharu, Kelantan, Malaysia
2.
Dentistry Department, Hilla University College, Babylon, Iraq
Arch Med Sci Civil Dis 2021; 6: e68–e74
Online publish date: 2021/06/30
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Introduction
Ciprofloxacin is a broad-spectrum antibiotic which can cause a chondrotoxic effect in the growing condyle. Researchers found that green tea showed significant health benefits for a large number of disorders. The objective of the present research was to investigate the preventive potential of green tea extract against mandibular chondrotoxicity induced by ciprofloxacin in juvenile Wistar rats.

Material and methods
Twenty juvenile male rats were used and divided into four equal groups, the saline/water (S/W), saline/green tea (S/G), ciprofloxacin/water (C/W) and ciprofloxacin/green tea (C/G) treated groups. On day 32 of age, all the animals in C/W and C/G treated groups were subcutaneously injected with ciprofloxacin as two subcutaneous injections of 600 mg/kg of body weight, eight hours apart, while the S/W and S/G groups were subcutaneously injected with saline. The S/G and C/G groups were intragastrically gavaged by green tea extract in an oral dose of 300 mg/kg/day, 8 days before the subcutaneous injection of saline or ciprofloxacin. On day 34, all the animals were anaesthetized, and the mandibular condyle samples were taken immediately and processed.

Results
In comparison with the C/W treated group, the C/G treated group showed a significant increase in the thickness of the mandibular condylar cartilage, cartilage cell number, glycosaminoglycan content, and Bcl-2 immune expression (p < 0.05).

Conclusions
This study provides the first evidence that green tea extract can significantly decrease the chondrotoxic effects of ciprofloxacin in a rat mandibular condyle cartilage model.

keywords:

antibiotics, mandibular condylar cartilage, Bcl-2, safranin O

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