eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

2/2018
vol. 43
 
Share:
Share:
more
 
 
abstract:
Clinical immunology

Basic inflammatory indices and chosen neutrophil receptors expression in chronic haemodialysed patients

Zbigniew Baj, Zbigniew Zbróg, Adam Szuflet, Sylwia Mańka, Piotr Bartnicki, Ewa Majewska

(Centr Eur Immunol 2018; 43 (2): 168-173)
Online publish date: 2018/06/30
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Aim of the study
End stage renal disease (ESRD) patients on chronic haemodialysis (HD) are immuno-compromised and prone to infection. Toll-like receptors (TLRs) play a role as both primary sensors of pathogen invasion and activators of inflammatory reaction. To test if the immune impairment in HD patients is connected with the defective expression of the neutrophil TLRs, we aimed to examine their expression and chosen inflammatory indices.

Material and methods
We tested CD14, TLR4, and TLR9 expressions on neutrophils using flow cytometry. Soluble CD14, C-reactive protein (CRP), and mannose-binding lectin (MBL) concentrations were tested using the ELISA method in 31 ESRD patients on chronic haemodialysis programs and in 17 healthy control subjects.

Results
Neutrophil TLR4 and TLR9 expressions did not differ significantly compared to the controls. The ESRD patients had markedly increased CRP and sCD14 levels alongside decreased MBL concentrations and neutrophil CD14 expression. The TLR4 expression correlated well with both TLR9 and CD14 neutrophil expressions; however, the increased CRP in the blood did not correlate with the MBL concentration or TLR expression.

Conclusions
The chronic program of haemodialysis and biochemical disorders in ESRD patients result in a low-grade chronic inflammation with no significant impact on the expression of neutrophil TLR4 and TLR9.

keywords:

inflammation, toll-like receptors, innate immunity, neutrophils, haemodialysis

references:
Vaziri ND, Pahl MV, Crum A, Norris K. (2012): Effect of uremia on structure and function of immune system. J Ren Nutr 22: 149-156.
Cohen G, Hörl WH (2012): Immune dysfunction in uremia—An update. Toxins 4: 962-990.
Betjes MG (2013): Immune cell dysfunction and inflammation in end-stage renal disease. Nat Rev Nephrol 9: 255-265.
Kim HW, Woo YS, Yang HN, et al. (2011): Primed monocytes: putative culprits of chronic low-grade inflammation and impaired innate immune responses in patients on haemodialysis. Clin Exp Nephrol 15: 258-263.
Kato S, Chmielewski M, Honda H, et al. (2008): Aspects of immune dysfunction in end-stage renal disease. Clin J Am Soc Nephrol 3: 1526-1533.
Piroddi M, Bartolini D, Ciffolilli S, Galli F (2013): Nondialyzable uremic toxins. Blood Purif 35 (Suppl. 2): 30-41.
Oleśkowska-Florek W, Połubinska A, Baum E, et al. (2014): Haemodialysis-induced changes in the blood composition affect function of the endothelium. Hemodial Int 18: 650-656.
Suresh R, Mosser DM (2013): Pattern recognition receptors in innate immunity, host defense, and immunopathology. Adv Physiol Educ 37: 284-291.
Eisen DP, Dean MM, Boermeester MA, et al. (2008) Low serum mannose-binding lectin level increases the risk of death due to pneumococcal infection. Clin Infect Dis 47: 510-516.
Prince LR, Whyte MK, Sabroe I, Parker LC (2011): The role of TLRs in neutrophil activation. Curr Opin Pharmacol 11: 397-403.
Hayashi F, Means TK, Luster A (2003): Toll-like receptors stimulate human neutrophil function. Blood 102: 2660-2669.
Kumar H, Kawai T, Akira S (2009): Toll-like receptors and innate immunity. Biochem Biophys Res Commun 388: 621-625.
Plitas G, Burt BM, Nguyen HM, et al. (2008): Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis. J Exp Med 205: 1277-1283.
Koc M, Toprak A, Arikan H, et al. (2011): Toll-like receptor expression in monocytes in patients with chronic kidney disease and haemodialysis: relation with inflammation. Nephrol Dial Transplant 26: 955-963.
Kuroki Y, Tsuchida K, Go I, et al. (2007): A study of innate immunity in patients with end-stage renal disease: special reference to toll-like receptor-2 and -4 expression in peripheral blood monocytes of haemodialysis patients. Int J Mol Med 19: 783-790.
Ando M, Shibuya A, Tsuchiya K, et al. (2006): Reduced expression of Toll-like receptor 4 contributes to impaired cytokine response of monocytes in uremic patients. Kidney Int 70: 358-362.
Vallés PG, Melechuck S, González A, et al. (2012): Toll-like receptor 4 expression on circulating leucocytes in hemolytic uremic syndrome. Pediatr Nephrol 27: 407-415.
Gollapudi P, Yoon JW, Gollapudi S, et al. (2010): Leukocyte toll-like receptor expression in end-stage kidney disease. Am J Nephrol 31: 247-254.
Silva SC, Baggio-Zappia GL, Brunialti MKC, et al. (2014): Evaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes. Braz
J Med Biol Res 47: 384-393.
Marcos V, Latzin P, Hector A, et al. (2010): Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases. Respir Res 11: 32.
Landmann R, Muller B, Zimmerli W (2000): CD14, new aspects of ligand and signal diversity. Microbes Infect 2: 295-304.
Akbari R, Mireskandari M, Alizadeh-Navaei R, Ghods A (2011): Measurement of serum levels of mannose-binding lectin in haemodialysis patients: a comparison with healthy individuals. Iran J Kidney Dis 5: 255-259.
Satomura A, Fujita T, Yanai M, et al. (2012): Functional mannose-binding lectin levels in patients with end-stage renal disease on maintenance haemodialysis. J Innate Immun 4: 293-300.
Ishii M, Ohsawa I, Inoshita H, et al. (2011): Serum concentration of complement components of the lectin pathway in maintenance haemodialysis patients, and relatively higher levels of L-Ficolin and MASP-2 in Mannose-binding lectin deficiency. Ther Apher Dial 15: 441-447.
Majewska E, Baj Z, Sulowska Z, et al. (2003): Effects of uraemia and haemodialysis on neutrophil apoptosis and expression of apoptosis-related proteins. Nephrol Dial Transplant 18: 2582-2588.
Lorenzen JM, David S, Richter A, et al. (2011): TLR-4+ peripheral blood monocytes and cardiovascular events in patients with chronic kidney disease – a prospective follow-up study. Nephrol Dial Transplant 26: 1421-1424.
FEATURED PRODUCTS
Quick links
© 2018 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe