eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
Current issue Archive Manuscripts accepted About the journal Editorial board Journal's reviewers Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
3/2021
vol. 38
 
Share:
Share:
more
 
 
abstract:
Original paper

Beta2-adrenergic receptor agonist inhibits keratinocyte proliferation by mechanisms involving nitric oxide

Chieh-Shan Wu
1
,
Der-An Tsao
2
,
Huoy-Rou Chang
3

1.
Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung city, Taiwan
2.
Department of Medical Technology, Fooyin University, Kaohsiung city, Taiwan
3.
Department of Biomedical Engineering, College of Medicine, I-Shou University, Kaohsiung city, Taiwan
Adv Dermatol Allergol 2021; XXXVIII (3): 396–403
Online publish date: 2020/02/11
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Introduction
Beta2-adrenoceptors regulate proliferation of keratinocytes. Nitric oxide (NO) produced by keratinocytes through stimulation of nitric oxide synthase (NOS) mediates keratinocyte proliferation.

Aim
In this study, the mechanism interaction β-ARs and NO production on keratinocyte will be explored, and the important for proliferation will be studied.

Material and methods
To understand the relationship among β2-adrenoceptors, NO production and proliferation in keratinocytes, the experiment is divided to two parts. In the first part of the experiment, keratinocytes are divided into five groups which are treated with 0 M, 10–7 M, 10–6 M, 5 × 10–6 M and 10–5 M isoproterenol, respectively. In the second part of the experiment, the keratinocytes are divided into five groups which are treated with 10–5 M isoproterenol and L-NMMA at doses of 0 M, 10–6 M, 5 × 10–6 M, 10–5 M and 5 × 10–5 M, respectively. We examine NOS expression, NO production, c-AMP level and proliferation in human keratinocytes.

Results
The results show that isoproterenol results in iNOS and ncNOS protein raised and the elevation of nitric oxide. L-NMMA can block the increase of iNOS and ncNOS protein expression and the ability to inhibit proliferation caused by isoproterenol.

Conclusions
Beta2-adrenergic receptor agonist mediates nitric oxide synthase to affect keratinocyte proliferation in skin. The physiological and pathological relationship of these discoveries remains to be defined. These results can provide new possibilities in the therapy of integumentary disease conditions linked with the dysfunction of β-AR-mediated NO production.

keywords:

β2-adrenoceptors, nitric oxide synthase (NOS), nitric oxide (NO), keratinocyte, proliferation

Quick links
© 2021 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe