eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
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SCImago Journal & Country Rank
3/2020
vol. 37
 
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abstract:
Original paper

Biochemical and histopathologic assessment of effects of acitretin on epiphyseal growth plate in rats

Sevda Onder
1
,
Serap Gunes Bilgili
2
,
Gulay Bulut
3
,
Huseyin Tugrul Celik
4
,
Serpil Oguztuzun
5
,
Hacı Onder
6
,
Omer Calka
2
,
Ayse Serap Karadag
7

1.
Department of Dermatology, Faculty of Medicine, Ordu University, Ordu, Turkey
2.
Department of Dermatology, Faculty of Medicine, Yuzuncu Yıl University, Van, Turkey
3.
Department of Pathology, Faculty of Medicine, Yuzuncu Yıl University, Van, Turkey
4.
Department of Biochemistry, Faculty of Medicine, Turgut Ozal University, Ankara, Turkey
5.
Department of Biology, Faculty of Arts and Sciences, Kırıkkale University, Kırıkkale, Turkey
6.
Department of Orthopaedics and Traumatology, Ordu University Training and Research Hospital, Ordu, Turkey
7.
Department of Dermatology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey
Adv Dermatol Allergol 2020; XXXVII (3): 346-352
Online publish date: 2020/07/16
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Introduction
Acitretin is a commonly used retinoid in dermatology. Although there are generally known side effects, the effects on the epiphyseal plaque and bone metabolism are not clear in the literature.

Aim
To histopathologically investigate the effects on the epiphyseal plate and assess variations in bone metabolism caused by acitretin.

Material and methods
Three groups were formed with 10 rats in each group. The 1st group (n = 10, 5 male, 5 female) were administered 10 mg/kg/day oral acitretin solution and the 2nd group (n = 10, 5 male, 5 female) were administered 3 mg/kg/day oral acitretin solution. The control group were given normal standard feed and water. Rats were sacrificed at the end of 4 weeks. The proximal tibias were excised and histopathologically and immunohistochemically assessed. Biochemical assessment was also carried out.

Results
Staining with haematoxylin-eosin found reductions in the epiphyseal plate in the 1st and 2nd group compared to the control group, though this situation was not statistically significant. Immunohistochemical studies did not encounter Type II collagen in the epiphyseal bone, proliferative zone and hypertrophic zone in the control group, low dose acitretin solution group and high dose acitretin solution group. Type II collagen was not observed in osteoids and osteoblasts. Type I collagen was not observed in the hypertrophic zone and proliferative zone of any group.

Conclusions
Our data show that though acitretin caused degeneration of the epiphyseal plate, it did not cause clear thinning and we identified no significant variations in bone metabolism markers.

keywords:

acitretin, epiphyseal plate, bone metabolism, rat

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