eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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4/2019
vol. 44
 
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abstract:
Clinical immunology

CD163 and CCR7 as markers for macrophage polarisation in lung cancer microenvironment

Iwona Kwiecień
,
Małgorzata Polubiec-Kownacka
,
Dariusz Dziedzic
,
Dominika Wołosz
,
Piotr Rzepecki
,
Joanna Domagała-Kulawik

(Centr Eur J Immunol 2019; 44 (4): 395-402)
Online publish date: 2020/01/20
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Introduction
M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF).

Aim of the study
To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-.

Material and methods
Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively).

Results
We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7lowCD163+), and cells with a stronger CCR7 (CCR7+CD163low). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7lowCD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163low). The median proportion of TGF-1 concentration was higher in the clBALF and hlBALF supernatant than in the serum.

Conclusions
In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer.

keywords:

M1 macrophages, M2 macrophages, bronchoalveolar lavage fluid, CCR7, TGF-1, lung cancer

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