ISSN: 2451-0629
Archives of Medical Science - Atherosclerotic Diseases
Current volume Archive About the journal Editorial board Abstracting and indexing Contact Instructions for authors Ethical standards and procedures
Official journal of the International Lipid Expert Panel (ILEP)
CALL FOR PAPERS
1/2020
vol. 5
 
Share:
Share:
more
 
 
abstract:
Experimental research

Cardioprotective effects of erythropoietin in diabetic rats determined by CD34 and vascular endothelial growth factor levels

Didem Melis Oztas
1, 2
,
Mert Meric
1
,
Metin Onur Beyaz
3
,
Mehmet Akif Onalan
4
,
Kivilcim Sonmez
5
,
Kerem Oter
5
,
Sedat Ziyade
6
,
Beyhan Omer
7
,
Ufuk Alpagut
1
,
Murat Ugurlucan
3

1.
Department of Cardiovascular Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
2.
Cardiovascular Surgery Clinic, Bagcilar Education and Research Hospital, Istanbul, Turkey
3.
Department of Cardiovascular Surgery, Medical Faculty, Medipol University, Istanbul, Turkey
4.
Department of Cardiovascular Surgery, Acibadem Mehmet Ali Aydinlar University, Atakent Hospital, Istanbul, Turkey
5.
Veterinary Faculty, University-Cerrahpasa, Istanbul, Turkey
6.
Department of Thoracic Surgery, Bezmialem University, Istanbul, Turkey
7.
Department of Biochemistry, Medical Faculty, Istanbul University, Istanbul Turkey
Arch Med Sci Atheroscler Dis 2020; 5: e1–e12
Online publish date: 2020/01/19
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Introduction
In this study, the effects of diabetes mellitus on the cardiovascular system were investigated by assessing the stem cell levels in serum and heart and compared with the normal population. Additionally, efficacy of erythropoietin, which is known to increase stem cells, was studied in diabetic rats.

Material and methods
Twenty-five male Sprague Dawley rats were divided into three groups as a control group (group 1), diabetic group (group 2) and erythropoietin induced diabetic group (group 3). A diabetes model was created with streptozocin. In group 3 rats received 3000 U/kg of erythropoietin. At the end of 1 month blood reticulocyte levels, degree of tissue fibrosis and immunohistochemical assessment of reliable stem cell markers, CD34 and vascular endothelial growth factor (VEGF), were analyzed.

Results
The increase in the blood glucose levels resulted in a significant decrease in reticulocyte levels in group 2. The increase in blood glucose levels resulted in a statistically significant increase in tissue level of fibrosis, CD34 and VEGF. When the rats in groups 1 and 2 were compared, the fibrosis, CD34 and VEGF levels were found to increase significantly. When group 2 and group 3 were compared, the amount of fibrosis was lower and the levels of CD34 and VEGF were significantly higher in group 3 than group 2.

Conclusions
The results of our study indicated that the amount of CD34 and VEGF which function in cellular protection and tissue regeneration may be enhanced with safely applicable erythropoietin leading to increase in reticulocyte levels in serum, and CD34 and VEGF levels in right atrium, right ventricle, left atrium, and left ventricle as a protective mechanism in diabetic rats.

keywords:

cardioprotective effect mechanisms, erythropoietin, diabetes mellitus

Quick links
© 2020 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe