eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2015
vol. 40
 
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abstract:

Clinical immunology
Prohibitin as a novel autoantigen in rheumatoid arthritis

Li-Li Shi
,
Peng Chen
,
Yi-Ping Xun
,
Wei-Kang Yang
,
Chun-He Yang
,
Guang-Yu Chen
,
Hong-Wu Du

(Centr Eur J Immunol 2015; 40 (1): 78-82)
Online publish date: 2015/04/22
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The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis.

Material and methods: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples.

Results: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%).

Conclusions: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients’ blood circulation.

keywords:

autoantibodies, autoantigen, autoimmune disease, prohibitin, rheumatoid arthritis

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