eISSN: 1896-9151
ISSN: 1734-1922
Archives of Medical Science
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SCImago Journal & Country Rank
5/2010
vol. 6
 
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abstract:

Clinical research
A survey on the distribution of healthy people with different anti-tumour ability

Yanhong Gao
,
Xiaolan Xu
,
Zhennan Dong
,
Chaoguang Jiang
,
Jin Gao
,
Jinchuan Hu
,
Junhao Gui
,
Haibao Wang
,
Yaping Tian

Arch Med Sci 2010; 6, 5: 806-814
Online publish date: 2010/10/27
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Introduction : The aim of the study was to explore the distribution of healthy people with different anti-tumour ability. Material and methods : Leukocytes were separated by the Ficoll-Hypaque density gradient centrifugal method. Then they were mixed with A549, MCF-7 and Hela cells at different ratios. The survival rate for target cells was observed and counted by Fluoroskan. Immune function for 200 healthy people was analysed by flow cytometry. Results : The results obtained by confocal microscopy revealed that human blood leukocytes possessed direct anti-tumour activity. The survival rate for tumour cells was the lowest in the condition of 20:1 ratio of effector cells to target cells. We speculated that in 200 healthy people the leukocyte capacity for killing MCF-7 cells is stronger than the leukocyte capacity for killing A549 cells and Hela cells. We also found that the distribution for 200 healthy people with different anti-tumour ability was different for different tumour cells. The number of healthy people with the strongest anti-tumour ability was highest when the target cells were MCF-7 cells. Moreover, the survival of A549, MCF-7 and Hela cells was correlated with T, B and NK lymphocytes. Conclusions : From the above, we can select healthy individuals with strong anti-tumour ability as anti-tumour donors according to their distribution with different anti-tumour ability, which opened up a new direction for fighting human cancer.
keywords:

innate immunity, tumour, leukocytes, polymorphonuclear leucocytes, distribution, healthy people

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