eISSN: 1896-9151
ISSN: 1734-1922
Archives of Medical Science
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5/2010
vol. 6
 
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abstract:

Clinical research
Altered transcriptional activity of gene encoding GAPDH in peripheral blood mononuclear cells from patients with cardiac syndrome X – an important part in pathology of microvascular angina?

Jozefa Dabek
,
Jakub Wilczok
,
Andrzej Kulach
,
Zbigniew Gasior

Arch Med Sci 2010; 6, 5: 709-712
Online publish date: 2010/10/27
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Introduction : Cardiac syndrome X (CSX) is characterized by anginal pain with ECG suggestive of ischaemia and normal coronary arteries at angiography. Pathology of CSX involves microvascular dysfunction and is possibly linked with metabolic syndrome. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. The GAPDH gene is a “housekeeping” gene and is used for normalization in quantitative gene expression assays. The aim of the study was to evaluate GAPDH gene expression in CSX.
Material and methods : The study was performed in 35 CSX patients and 10 con­trol subjects. mRNA was extracted from peripheral blood mononuclears and the mRNA was assessed by QRT-PCR.
Results : GAPDH gene expression was enhanced in CSX patients vs. controls (93022 ±23837 copies/μg vs. 1067 ±240 copies/μg respectively; p < 0.001). Moreover, transcriptional activity of the GAPDH gene was heterogeneous within the CSX group.
Conclusions : GAPDH gene expression is markedly enhanced in CSX, which reflects carbohydrate metabolism disturbances and makes the GAPDH gene unsuitable as an endogenous control in patients with CSX.
keywords:

GAPDH, cardiac syndrome X, gene expression

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