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vol. 11

Clinical research
Arthroscopic posterior cruciate ligament reconstruction with allograft versus autograft

Xiujiang Sun
Jianfeng Zhang
Xiaoyi Qu
Yanping Zheng

Arch Med Sci 2015; 11, 2:395–401
Online publish date: 2015/04/23
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The posterior cruciate ligament (PCL) plays a very important role in the posterior stability and function of the knee joint. Posterior cruciate ligament injuries occur in up to 44% of all knee injuries [1], but isolated PCL injuries have a reported incidence of 3.5% [2]. Posterior cruciate ligament injuries often appear in sport injuries or high-energy trauma [3–6]. The treatment of the PCL injury is controversial, although the instrument and technique have been improved greatly. There are documents reporting the encouraging management of isolated PCL tears [7], but PCL deficiency can cause severe instability of the knee and degeneration of the knee [8]. Posterior cruciate ligament reconstruction receives wide interest in the orthopedic literature [9].
The graft choice is another question we need to consider. Artificial ligaments have shown poor clinical outcomes due to loosening, fatigue failure, and a strong host immune response [10, 11]. Many studies have reported that bone-patellar-bone and hamstring autografts can produce good clinical outcomes in ligament reconstruction [12, 13]. However, autografts may cause greater surgical trauma and postoperative donor-site morbidity. The hamstring autograft harvested can cause weakening of the flexor apparatus [14]. The use of allografts can minimize the operative trauma and eliminate the donor-site morbidity [15]. Also, it is more appropriate for revision surgery and multiple ligament injury [16]. Several studies have compared allografts with autografts in anterior cruciate ligament (ACL) reconstruction. The results show that the allograft is a reasonable alternative to the autograft [17, 18]. The allograft tissue has its own problems, such as disease transmission, bacterial, viral, and host immune response, delayed incorporation, and bone tunnel enlargement [19–22].
The purpose of our studies was to compare the outcomes of PCL reconstruction using autografts and allografts retrospectively. Our hypothesis was that the clinical results of PCL reconstruction using allografts were equivalent to those using autografts.

Material and methods

Patients selection

The study was a retrospective observational analysis. This study compared the outcomes of primary PCL reconstructions with autografts or allografts from April 2005 to July 2009. Exclusion criteria included ACL injury combined, vascular and neural complications, collateral ligament injury (grade III and above), large cartilage lesion (Outerbridge grade III or IV larger than 1.0 cm in diameter) and so on. The patients with meniscus injury combined were not excluded. Seventy-one patients met our inclusion criteria. Ten patients were excluded due to ACL injury combined (3), common peroneal nerve injury (1), grade III collateral ligaments injury (4), and large cartilage lesion (2).
The diagnosis was confirmed through orthopedic examination and preoperative magnetic resonance imaging (MRI). The orthopedic examination and MRI have an important role in diagnosis [23]. All the patients had a symptomatic isolated PCL rupture with the three-plus posterior drawer test. All the patients had significant posterior instability. All procedures were performed by the same surgeon with the same technique, but the surgeon did not take part in the rehabilitation process. Graft selection was based on patient preference. All rehabilitations were the same and were performed by the same physician.

Harvest and preparation of grafts

We harvested the autogenous hamstring (semitendinosus and gracilis) tendon from the injured side. After the tendons were folded over at their centers, the diameter should have been at least 8 mm. Otherwise, those of the uninjured side were harvested. All allogeneic tendons were supplied by a certified tissue bank. The allogeneic tendon was sterilized with gamma irradiation at a dose of 2.5 mrad. The allogeneic tendon was dipped in physiological saline with gentamicin. Both the tendons were folded over at their centers, creating a four-strand graft (Figure 1). The folded end was fixed in a femoral tunnel. The four ends of the four-strand tendon were sutured with ‘2’ polyester whipstitch-style sutures separately. The diameter was 8–9 mm. The length was at least 12 cm. All tendons were prepared in an identical fashion, and the grafts were pretensioned under 20 lb for 20 min.

Surgical technique

We made the standard anterolateral and anteromedial portals. Firstly, the preliminary diagnosis arthroscopy was performed, in order to find any meniscal lesions and other injuries. Once the meniscal lesions were found, the partial meniscectomy or repair would be performed. After that, the posteromedial portal was made so as to find the rupture of the PCL and localize the exit point of the tibia tunnel. During the operation process, if the PCL remnant is observed, it should be preserved, because it is helpful to obtain successful outcomes (Figure 2). The exit point was 1.5 cm below the joint surface, lateral to the midline in the posterior recess of the tibia. The tunnel size should match the graft diameter. When drilling the tunnel, one should take care not to damage the popliteal vessels.
The femoral tunnel centre was 6 mm to 8 mm from the distal articular surface with the same opening measuring as the graft diameter. It was placed at the half-past-one or half-past-ten position according to the side (Figure 3). After the graft was passed through the tibia tunnel and then into the femoral tunnel, its femoral end was fixed with absorbable interference screws (DePuy Mitek). At the knee 70° flexed and tibia anterior drawer position, the tibia end was fixed with absorbable interference screws (DePuy Mitek) after moving the knee 20 times through a full range of motion.

Postoperative rehabilitation

Static quadriceps and straight leg-raising exercises were started at once after the operation. All patients were non-weight-bearing for 6 weeks. A hinged brace was necessary for the patient in the first 2 months. The knee was braced in full extension for 2 weeks. From the third week, the knee passive flexion began twice a day. A 90° range of flexion was allowed after 4 weeks postoperatively. The knee flexion was limited to 0–120° after 3 months postoperatively and 0–140° after 4 months postoperatively. Six weeks postoperatively the patient could walk with full weight bearing. Four months postoperatively, a progressive running program was initiated. After 10 postoperative months, contact sports activities were allowed.


The postoperative assessments were performed at the follow-up point (1, 2, 4, 6, 9, and 12 months and then yearly) to obtain measures of the clinical outcomes. The operation time, incision length, postoperative fever time (when the axillary temperature is more than 37.2°C), infection rate and routine blood test were compared after the operation. The incidence of numbness and dysesthesia around the incision were also compared. The posterior drawer test and the KT2000 test were used to evaluate the posterior stability of knee joints. The posterior laxity was assessed by manual-maximum KT-2000 tests at the knee positioned with 90° flexion. Side-to-side differences in posterior laxity were determined. Functional tests included the range of motion (ROM), international Knee Documentation Committee standard evaluation form (IKDC), Lysholm scores and Tegner activity score. To minimize bias, all evaluations were performed by the same investigator, who did not take part in our study. A routine blood test was performed preoperatively and 1 day postoperatively to compare the number of white blood cells, neutrophilic granulocytes and other cells.

Statistical analysis

SPSS 13.0 was used for statistical analysis. The independent-samples and paired-samples T-tests were used for the comparison of continuous variables, and the 2 test was used for categorical variables. The significance level was set at p ≤ 0.05.


Seventy-one patients (36 patients in the autograft group and 35 patients in the allograft group) met our inclusion criteria (Table I). The average follow-up of the autograft group was 3.2 ±0.2 years and that of the allograft group was 3.3 ±0.6 years, showing no difference (p = 0.764).
The average duration of the autograft procedure was longer than that of the allograft procedure. There was a difference between the two groups in fever time (when axillary temperature is more than 37.2°C) and incision length. Patients in the autograft group had a longer incision and a shorter postoperative fever time (Table II).
The combined injuries included meniscus injury, cartilage lesion and media collateral ligament injury. Repair or partial meniscectomy was performed for the meniscus injury. Debridement was performed for cartilage lesion. No treatment was performed for the media collateral ligament (Table III).
A significant difference was found in the posterior drawer test between the two groups at the final follow-up. The KT-2000 test results also showed a difference between the two groups in the tibia posterior displacement and in the average manual maximum KT-2000 side-to-side difference in posterior tibial displacement at 90° knee flexion (Table IV).
Arthrofibrosis occurred in 3 patients in the autograft group and 4 patients in the allograft group. After physical therapy, the patient’s knee ROM was improved greatly, without any impact on the final evaluation. No loss of flexion or extension was observed requiring manipulation and no debridement developed postoperatively in any patient in either group. There were 8 patients in the autograft group with leg muscle thrombosis and 7 patients in the allograft group, cured with conservative treatments. There was no significant difference between the two groups (p = 0.614). There was no infection in either group.
Numbness and dysesthesia around the incision were present in 22 patients in the autograft group (61%), in contrast to 10 patients in the allograft group (29%) (p = 0.006). The area of numbness and dysesthesia was located at the anteromedial and anterolateral aspect of the knee mainly.
At the final follow-up, the average ROM was 132.3 ±2.2° in the autograft and 134.6 ±1.8° in the allograft group, showing no significant difference (p = 0.875). The IKDC objective scale, Lysholm score and Tegner activity score showed greater improvement at the final follow-up compared to preoperation (p = 0.006, 0.001, 0.005). There were no significant differences between the two groups at the final follow-up (Table V).
In both groups, the white blood cells (WBC), neutrophils, neutrophil% and lymphomonocytes increased after surgery (p = 0.036, 0.033, 0.029, 0.040 in the autograft group and p = 0.034, 0.027, 0.021, 0.039 in the allograft group). Also, the changes of the WBC and neutrophils in the allograft group were more significant than those in the autograft group (p = 0.026, 0.035). The lymphomonocyte% remained stable after surgery compared to before in both groups. The mean number of leukomonocytes and percentage of leukomonocytes highly decreased after surgery (p = 0.031, 0.039) (Table VI).


Graft choice still remains controversial [24]. Which is best? In recent years there have been many reports about this topic, but the findings vary greatly [25–27]. Many studies have reported good clinical results using allografts for ligament reconstructions [28–31]. Compared to the autograft, the allograft has many advantages including minimizing postoperative donor-site morbidity, no weakening of the flexor apparatus, shorter operation time, and so on. The aim of our study was to compare the outcomes of arthroscopic posterior cruciate ligament reconstruction with different grafts. In our study, 71 patients underwent arthroscopic single bundle PCL reconstruction with an autograft or allograft. There was no difference between the two groups according to the IKDC, Lysholm score and Tegner activity score at the final follow-up. But the instrumented posterior laxity in the allograft group increased more than that in the autograft group. We also found that the incidence of numbness and dysesthesia around the incision in the autograft group was higher (61% in autograft versus 28% in allograft group). Graft selection is a very important part of our preoperative planning. Our selection depended on the patients’ preference, ages, expected outcomes, tissue availability and other factors.
Prodromos et al. reported that irradiated grafts had an abnormal stability rate of 31% vs. 12% for non-irradiated grafts [32]. In our study, the allogeneic tendons we used were sterilized with gamma irradiation at a dose of 2.5 mrad, by taking account that much gamma irradiation can greatly alter the initial biomechanical properties of allografts in a dose-dependent manner [33–35]. The gamma irradiation may change the biomechanical properties. The change of the biomechanical properties may lead to posterior laxity. Other papers have reported that the enlarged tunnel was partly responsible for the laxity [36, 37].
The sensory disturbance postoperatively was mainly located at the anteromedial and anterolateral aspect of the knee, where the infrapatellar branch of the saphenous nerve was supplied. Since the nerve is found immediately superficial to the gracilis tendon [38], the hamstring harvesting could damage the branches of the saphenous nerve [39].
We performed a routine blood test for every patient 1 day postoperatively. We found that the numbers of neutrophilic granulocytes and white blood cells in the allograft group essentially increased postoperatively in comparison to those in the autograft group. It is possible that a relationship exists between the elevation of WBC and postoperative fever time, but this needs further evidence-based tests.
There were some limitations in our study. First, the follow-up was not very long, so some long-term outcomes could not be observed. Second, the group of patients was relatively small, and a post hoc power analysis was not performed; its statistical power would be affected. Third, the graft selection was performed based on patients’ preference. Fourth, the routine blood test was only performed 1 day postoperatively, without indicating the trend of changes of blood cells. The relationship between the changes of blood routine parameters and the postoperative symptoms still needs further investigation.
In conclusion, both groups of patients had satisfactory clinical outcomes after the operation. There were no differences in functional test results between the 2 groups. However, in the instrumented posterior laxity test, the autograft gave better results than the allograft.

Conflict of interest

The authors declare no conflict of interest.


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