eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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vol. 42
Experimental immunology

Differentiated all-trans retinoic acid response of naive CD4+CD25– cells isolated from rats with collagen-induced arthritis and healthy ones under in vitro conditions

Isaura Felcenloben
Edyta Żyromska
Tomasz Piasecki
Joanna Rossowska
Anna Kędzierska
Marcin Nowak
Marcin Żyromski
Anna Chełmońska-Soyta

(Cent Eur J Immunol 2016; 41 (4): 39-53)
Online publish date: 2017/05/08
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Aim o the study: To compare the potential of CD4+CD25– cells, isolated from both healthy rats and rats with CIA (Collagen-Induced Arthritis), for differentiation into regulatory T cells in the presence of all-trans retinoic acid in order to learn more about the activation mechanisms and therapeutic potential of regulatory T cells.

Material and methods: Sorted CD4+CD25– cells were cultured in vitro with/without ATRA, and then the frequency of regulatory T cells and their ability to secrete IL-10 by CD4+ FOXP3+ cells was examined. Gene expression of the foxp3, rarα, rarβ, rxrβ, and ppar β/γ and protein expression of the Rarα, Rarβ, and Rxrβ in cells after stimulation with ATRA were also investigated.

Results: CD4+CD25– cells isolated from healthy animals or from animals with CIA are characterised by different potential of the differentiation into CD4+CD25+ FOXP3+ cells. Retinoic acid receptor Rxrβ is present in the CD4+CD25– cells isolated from rats with CIA.

Conclusions: We showed that although ATRA did not increase the frequency of Treg in culture, it significantly increased expression of rarα and rxrβ only in lymphocytes taken from diseased animals and foxp3 expression only in healthy animals. Moreover, after ATRA stimulation, the frequency of Treg-produced IL-10 tended to be lower in diseased animals than in the healthy group. The results imply that the potential of naïve cell CD4 lymphocytes to differentiate into Tregs and their putative suppressive function is dependent on the donor’s health status.

regulatory T cells, Treg, all-retinoic acid, atra, collagen-induced arthritis

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