Effect of oral administration of kynurenic acid on the activity of the peripheral blood leukocytes in mice

Joanna Małaczewska; Andrzej Krzysztof Siwicki; Roman Marcin Wójcik; Edyta Kaczorek; Waldemar Andrzej Turski

doi:10.5114/ceji.2014.42115

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors

Editorial System

Submit your Manuscript

1/2014
vol. 39

Send email

Copy url:

abstract:

Effect of oral administration of kynurenic acid on the activity of the peripheral blood leukocytes in mice

Joanna Małaczewska

,

Andrzej Krzysztof Siwicki

,

Roman Marcin Wójcik

,

Edyta Kaczorek

,

Waldemar Andrzej Turski

(Centr Eur J Immunol 2014; 39 (1): 6-13

DOI: https://doi.org/10.5114/ceji.2014.42115

Online publish date: 2014/04/17

View full text Get citation

PlumX metrics:

Kynurenic acid (KYNA), an endogenous tryptophan metabolite, is a selective ligand of the GPR35 receptor, expressed mainly on the immune cells. In inflammatory conditions, by affecting this receptor, KYNA inhibits the synthesis of pro-inflammatory cytokines and probably protects tissues from oxidative damage. However, we lack data regarding the effect of exogenous KYNA on the activity of immune cells in healthy individuals. The objective of this study has been to determine the influence of kynurenic acid administered to mice in different doses (2.5, 25 or 250 mg/l) and for different time periods (3, 7, 14, 28 days) in drinking water, on the activity of their peripheral blood leukocytes. The determinations comprised the proliferative activity of lymphocytes (MTT assay) and the phagocytic activity as well as the respiratory burst activity of granulocytes and monocytes (Phagotest, Phagoburst). It was only the lowest KYNA dose that influenced the mitogenic response of lymphocytes, namely by increasing the proliferation of T cells. The impact on the phagocytic activity was varied with KYNA dose and administration time. However, all the KYNA doses significantly lowered the activity of oxidative burst in phagocytes, which was probably associated with its antioxidant properties. In the light of the research results, kynurenic acid may find applications as an immuno-modulating agent able to correct an excessive or insufficient response of phagocytizing cells, protecting an organism from oxidative stress.

keywords:

Effect of oral administration of kynurenic acid on the activity of the peripheral blood leukocytes in mice

Joanna Małaczewska , Andrzej Krzysztof Siwicki , Roman Marcin Wójcik , Edyta Kaczorek , Waldemar Andrzej Turski

kynurenic acid, proliferative response, oxidative burst, antioxidant properties, phagocytic activity

Joanna Małaczewska

,

Andrzej Krzysztof Siwicki

,

Roman Marcin Wójcik

,

Edyta Kaczorek

,

Waldemar Andrzej Turski