Abstract
Effects of lactoferrin on viral replication in B cell lines corresponding to various stages of B cell differentiation
- Department of Experimental Therapy, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
Introduction
Lactoferrin inhibits viral replication in many cell types.
Aim
The aim of this study was to evaluate the protective effects of lactoferrin (LF) on susceptibility of B cell lines, representing different stages of differentiation, to viral infection.
Material and methods
Native bovine LF (bLF) and recombinant mouse LF (rmLF) were applied. Mouse cell lines: WEHI-231 (immature B cells), A-20 (mature B cells), and 7TD1 (plasma cells) were used for infection with encephalomyocarditis virus (EMCV), herpes virus type 1 (HSV-1), and vesicular stomatitis virus (VSV).
Results
A-20 cells were most sensitive to infection, WEHI-231 cells were less sensitive, while 7TD1 cells were most resistant to the infection. Both lactoferrins showed significant protective effects in WEHI-231 cells against EMCV, and in the case of A-20 cell line this effect was strongest. A potent antiviral effect of the lactoferrins was found in A-20 cells infected with HSV-1.
Conclusions
The susceptibility of B-cell lines to viral infection, at various stage of cell differentiation, was differential. The protective effects of rmLF were higher in comparison to bLF.
>Keywords
lactoferrin, A-20, WEHI-231, 7TD1, EMCV, HSV-1, VSV
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