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abstract:
Experimental research

Ethyl pyruvate treatment ameliorates pancreatic damage: evidence from a rat model of acute necrotizing pancreatitis

Serdar Turkyilmaz, Arif Burak Cekic, Arif Usta, Etem Alhan, Birgül Vanizor Kural, Cengiz Ercin, Kutay Sağlam

Arch Med Sci
Online publish date: 2017/01/23
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Introduction: Ethyl pyruvate (EP), a natural flavoring and fragrance agent, has been shown to exert anti-inflammatory and antioxidant actions. We tested the potential beneficial effects of EP in a rat model of acute necrotizing pancreatitis (ANP), a serious condition with a significant inflammatory explosion and oxidative stress.

Material and methods: Fifty-two adult male Sprague-Dawley rats were divided into four groups: sham + saline, sham + EP, ANP + saline, and ANP + EP. The ANP was induced by glycodeoxycholic acid and cerulein. Animals were sacrificed at 48 h and biochemical, hematological, and histological markers of ANP and inflammation were assessed. The extent of mortality, systemic cardiorespiratory variables, pancreatic microcirculation, renal/hepatic functions, acinar cell injury and enzyme markers for pancreas and lung tissues were investigated.

Results: The EP-treated ANP group presented significantly lower mortality than the untreated ANP group (44% (7/16) vs. 19% (3/16), respectively, p < 0.05). Administration of EP resulted in significantly lower levels of IL-6 (ANP + saline: 5470 ±280 vs. ANP + EP: 2250 ±180 pg/ml, p < 0.05). Compared with the ANP group, the ANP + EP group had a lower pancreatic necrosis score (1.45 ±0.2 vs. 0.96 ±0.2, p < 0.05). Moreover, intraperitoneal EP administration had a positive effect on most indices of pancreatitis (amylase and alanine transaminase levels) and lung damage (except lung malondialdehyde levels) as they decreased towards baseline values.

Conclusions: The results from this experimental study indicate that EP, a nontoxic chemical approved by the Food and Drug Administration as a food additive, provides positive effects on the course of pancreatitis, suggesting potential usefulness in management of ANP.
keywords:

pancreatitis, rat, ethyl pyruvate, acute necrotizing pancreatitis

references:
Cruz-Santamaría DM, Taxonera C, Giner M. Update on pathogenesis and clinical management of acute pancreatitis. World J Gastrointest Pathophysiol 2012; 3: 60-70.
Whitcomb DC. Clinical practice. Acute pancreatitis. N Engl J Med 2006; 354: 2142-50.
Klar E, Messmer K, Warshaw AL, Herfarth C. Pancreatic ischemia in experimental acute pancreatitis: mechanism, significance and therapy. Br J Surg 1990; 77: 1205-10.
Qu Z, Jiang Y, Wu BQ, et al. Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats. Arch Med Sci 2014; 10: 825-9.
Beger HG, Bittner R, Büchler M, Hess W, Schmitz JE. Hemodynamic data pattern in patients with acute pancreatitis. Gastroenterology 1986; 90: 74-9.
Rau B, Poch B, Gansauge F, et al. Pathophysiologic role of oxygen free radicals in acute pancreatitis: initiating event or mediator of tissue damage? Ann Surg 2000; 231: 352-60.
Abogresha NM, Greish SM, Abdelaziz EZ, Khalil WF. Remote effect of kidney ischemia-reperfusion injury on pancreas: role of oxidative stress and mitochondria lapoptosis. Arch Med Sci 2016; 12: 252-62.
Faiber JL, Kyle ME, Coleman JB. Mechanisms of cell injury by activated oxygen species. Lab Invest 1990; 62: 670-9.
Stanfey H, Bulkley GB, Cameron JL. The role of oxygen-derived free radicals in the pathogenesis of acute pancreatitis. Ann Surg 1984; 200: 405-13.
Schoenberg MH, Birk D, Beger HG. Oxidative stress in acute and chronic pancreatitis. Am J Clin Nutr 1995; 62: 1306-14.
Jeurnink SM, Nijs MM, Prins HA, Greving JP, Siersema PD. Antioxidants as a treatment for acute pancreatitis: a meta-analysis. Pancreatology 2015; 15: 203-8.
Dobsak P, Courdertot-Masuyer C, Zeller M, et al. Antioxidative properties of pyruvate and protection of the ischemic rat heart during cardioplegia. J Cardiovasc Pharmacol 1999; 34: 651-9.
Luan ZG, Zhang H, Ma XC, Zhang C, Guo RX. Role of high-mobility group box 1 protein in the pathogenesis of intestinal barrier injury in rats with severe acute pancreatitis. Pancreas 2010; 39: 216-23.
Fink MP. Ethyl pyruvate: a novel anti-inflammatory agent. J Intern Med 2007; 261: 349-62.
Sims CA, Wattanasirichaigoon S, Menconi MJ, Ajami AM, Fink MP. Ringer’s ethyl pyruvate solution ameliorates ischemia/reperfusion induced intestinal mucosal injury in rats. Crit Care Med 2001; 29: 1513-8.
Yang R, Shaufl AL, Killeen ME, Fink MP. Ethyl pyruvate ameliorates liver injury secondary to severe acute pancreatitis. J Surg Res 2009; 153: 302-9.
Dobschuetz E, Biberthaler P, Mussack T, Langer S, Messmer K, Hoffmann T. Noninvasive in vivo assessment of the pancreatic microcirculation: orthogonal polarization spectral imaging. Pancreas 2003; 26: 139-43.
Groner W, Winkelman JW, Harris AG, et al. Orthogonal polarization spectral imaging: a new method for study of the microcirculation. Nat Med 1999; 5: 1209-12.
Schmidt J, Rattner MD, Lewandrowski K. A better model of acute pancreatitis for evaluating therapy. Ann Surg 1992; 215: 44-56.
Sookhal S, Wang JJ, McCourt M, Kwan W, Bouchier DH, Redmond P. A novel therapeutic strategy for attenuating neutrophil-mediated lung injury in vivo. Ann Surg 2002; 235: 283-91.
Lankisch PG, Pohl U, Otto J, Rahlf G. When should treatment of acute experimental pancreatitis be started? The early phase of bile -induced acute pancreatitis. Res Exp Med 1988; 188: 123-9.
Schierwagen C, Bylund-Fellenius AC, Lundberg C. Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods 1990; 23: 179-86.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-10.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.
Riedemann NC, Guo RF, Ward PA. Novel strategies for the treatment of sepsis. Nat Med 2003; 9: 517-24.
Bennett-Guerrero E, Swaminathan M, Grigore AM, et al. A phase II multicenter double-blind placebo-controlled study of ethyl pyruvate in high-risk patients undergoing cardiac surgery with cardiopulmonary bypass. J Cardiothorac Vasc Anesth 2009; 23: 324-9.
Cheng BQ, Liu CT, Li WJ, et al. Ethyl pyruvate improves survival and ameliorates distant organ injury in rats with severe acute pancreatitis. Pancreas 2007; 35: 256-61.
Yang ZY, Ling Y, Yin T, et al. Delayed ethyl pyruvate therapy attenuates experimental severe acute pancreatitis via reduced serum high mobility group box 1 levels in rats. World J Gastroenterol 2008; 14: 4546-50.
Luan ZG, Zhang H, Ma XC, Zhang C, Guo RX. Therapeutic treatment with ethyl pyruvate attenuates the severity of liver injury in rats with severe acute pancreatitis. Pancreas 2012; 41: 729-37.
Luan ZG, Ma XC, Zhang H, Zhang C, Guo RX. Protective effect of ethyl pyruvate on pancreas injury in rats with severe acute pancreatitis. J Surg Res 2013; 181: 76-84.
Yang CJ, Chen J, Phillips AR, Windsor JA, Petrov MS. Predictors of severe and critical acute pancreatitis: a systematic review. Dig Liver Dis 2014; 46: 446-51.
Leung PS, Chan YC. Role of oxidative stress in pancreatic inflammation. Antioxid Redox Signal 2009; 11: 135-65.
Kung CW, Lee YM, Yen MH. In vivo anticoagulant effect of ethyl pyruvate in endotoxemic rats. Thromb Res 2011; 127: 582-8.
Crawford RS, Albadawi H, Atkins MD, et al. Postischemic treatment with ethyl pyruvate prevents adenosine triphosphate depletion, ameliorates inflammation, and decreases thrombosis in a murine model of hind-limb ischemia and reperfusion. J Trauma 2011; 70: 103-10.
van Zoelen MA, Bakhtiari K, Dessing MC, et al. Ethyl pyruvate exerts combined anti-inflammatory and anticoagulant effects on human monocytic cells. Thromb Haemost 2006; 96: 789-93.
Zhang X, Wu D, Jiang X. ICAM-1 and acute pancreatitis complicated by acute lung injury. JOP 2009; 10: 8-14.
Murakami H, Nakao A, Kishimoto W, Nakano M, Takagi H. Detection of O2- generation and neutrophil accumulation in rat lungs after acute necrotizing pancreatitis. Surgery 1995; 118: 547-54.
Matone J, Moretti AI, Apodaca-Torrez FR, Goldenberg A. Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis. Acta Cir Bras 2013; 28: 559-67.
Papachristou GI. Prediction of severe acute pancreatitis: current knowledge and novel insights. World J Gastroenterol 2008; 14: 6273-5.
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