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Experimental research

Ethyl pyruvate treatment ameliorates pancreatic damage: evidence from a rat model of acute necrotizing pancreatitis

Serdar Turkyilmaz
,
Arif Burak Cekic
,
Arif Usta
,
Etem Alhan
,
Birgül Vanizor Kural
,
Cengiz Ercin
,
Kutay Sağlam

Arch Med Sci 2019; 15, 1: 232–239
Online publish date: 2017/01/23
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Introduction

Acute pancreatitis, a sudden onset inflammatory condition of the pancreas, is a mild and self-limiting disease that usually resolves spontaneously or by simple supportive therapy in most patients. However, in about 10–20% of patients, the course of acute pancreatitis can take the severe or necrotizing form, which generally requires prolonged periods of intensive care hospitalization with high costs, and significant morbidity and mortality rates. Despite recent improvements in medical management and care, the mortality of acute necrotizing pancreatitis (ANP) is still about 6.2% to 20.8%, and when the pancreas is infected this rate may be exceeded, largely due to sepsis and multiorgan failure [1, 2].

Pancreatic damage occurs by autodigestion of the pancreas when pancreatic enzymes are activated before being released into the intestine. As a result of attack of own tissue by these enzymes, pancreatic damage and impairment in pancreatic microcirculation are the typical two main events in the process of acute pancreatitis [1, 2]. The symptoms of acute pancreatitis may range from mild abdominal discomfort to severe pain, and at times this debilitating disease may become severe and life-threatening.

Experimental and clinical data indicate that the release of inflammatory mediators and subsequent activation of cytokines and collection of secondary mediators such as histamine, thromboxanes, prostaglandins, nitric oxide, leukotrienes, and platelet-activating factor from the pancreas and from various other sources play an important role in the course of acute pancreatitis leading to tissue destruction [1, 3, 4]. In the early phase, the disease is accompanied by hypovolemia, manifesting as hypotension and tachycardia, resulting from fluid sequestration into the abdominal cavity. If the resolution fails to occur, infection of pancreatic necrosis usually develops by translocation of enteric bacteria, and this may lead to multiorgan failure [1, 5].

Accumulating evidence indicates that reactive oxygen species (ROS) contribute to the pathogenesis of acute pancreatitis [6] locally in the pancreas and remote organs, both in the early and late stages. The exact site and source of enhanced ROS generation in the initial stages of this disease are not clear. In later stages, the attraction and activation of leukocytes in the pancreas cause enhanced ROS production [7]. It is well documented that ROS directly attack cell membrane lipids and proteins and disrupt their functions. The ROS also exert indirect actions, through action on the arachidonic metabolism, on these membrane structures [8]. The latest effect involves stimulation of increased secretion of thromboxane, which decreases tissue circulation due to its platelet aggregating and vasoconstricting actions. The ROS also cause increased production of leukotriene B4, which enhances the activation of leukocytes and the release of lysosomal enzymes [8]. As a vicious cycle, these processes lead to further cell damage of the pancreas. There is also experimental and clinical evidence on the role of ROS in acute pancreatitis [6, 9, 10]. Different experimental models and clinical studies indicate that antioxidants and free radical scavengers including melatonin provide significant beneficial effects on pancreatitis [11].

Pyruvate, the anionic form of 2-oxo-propionic acid, is the final product of glycolysis before entering the tricarboxylic acid cycle. Pyruvate is also a scavenger of ROS such as hydroxyl radical [12]. Ethyl pyruvate (EP) is an endogenous derivative of pyruvate. EP is known to exert more effective anti-oxidative and anti-inflammatory actions [13, 14]. There is experimental evidence that EP significantly protects against ischemia/reperfusion-induced intestinal mucosal injury, and also prevents liver injury secondary to ANP [15, 16]. Although previous experimental studies [15, 16] have successfully documented that EP ameliorates distant organ injury and reduces mortality in experimental ANP, they did not evaluate pancreatic microcirculation in detail. It is well documented that impairment of pancreatic microcirculation plays a key role in the pathogenesis of ANP. Considering the fact that microcirculation plays a crucial role in both physiological and pathophysiological states including ANP, analysis of microcirculation of the pancreas by orthogonal polarization imaging a video microscope provides a unique perspective to document impairment and assess possible effects of therapy on pancreatic microcirculation [17, 18]. This method has been successfully validated in a wide spectrum of experimental and limited clinical applications for assessment of functional capillary microcirculation [17, 18].

The aim of this study was to investigate possible effects of EP on ANP in a rat model of acute pancreatitis. Effects of EP on the parameters of extent of acinar cell damage, functional microcirculatory parameters (functional capillary density – FCD), systemic cardiorespiratory variables, evaluation of hepatic and renal functions, changes in some enzyme markers for pancreas and lung tissue, and mortality rates were investigated.

Material and methods

Animals

Fifty-two male Sprague Dawley rats (300–350 g) were used. The animals were housed in standard conditions (21 ±1ºC and a 12:12-h light/dark cycle) with free access to water. During the study period the animals were housed in metabolic cages, one in each cage, which enabled quantitative assessment of urine output. The protocol of this study was approved by the Ethics Committee of Karadeniz Technical University (Trabzon, Turkey, protocol number: 2009/11, date: 12.05.2009).

Experimental procedures

After overnight fasting, animals were anesthetized using vaporized ether for induction and intraperitoneal ketamine injection at 50 mg/kg (Ketalar, Eczacıbası, Istanbul, Turkey) for the maintenance of surgical anesthesia. For infusion, blood sampling and hemodynamic measurements the right internal jugular vein and carotid artery were cannulated (Luer Lock, ID 0.5 mm, Braun AG, Melsungen, Germany). The catheters were tunneled subcutaneously to the suprascapular area.

Acute pancreatitis was induced as previously described by Schmidt et al. [19]. Briefly, cerulein (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was given by intravenous infusion at a dose of 5 mg/kg/h over 6 h superimposed on a standard infusion of 1.2 ml/kg glycodeoxycholic acid (10 mmol/l, Sigma, St. Louis, USA) into the biliary-pancreatic duct for 10 min at 30 mm Hg through an infusion pump for pressure and volume control (Hampshire, United Kingdom, Alaris Medical Systems, RG22, 4BS, IVAC P 7000). For the baseline hydration, saline containing cerulein was infused at 8 ml ml/kg/h. The animals in the sham group were given intraductal saline followed by a 6-h intravenous saline infusion.

Rats were randomly divided into four groups (Figure 1). Group I (sham + saline, n = 10) received intraductal saline followed by a 6-h intravenous infusion of saline at 8 ml/kg/h. During the following 6-h period 1 ml of saline was given intravenously, and saline was infused (6 ml/kg, i.v.) for the last 18 h. At 24 h after the beginning of the experiment, the cardiorespiratory function was assessed by monitoring the arterial blood gases, mean arterial pressure (MAP), renal function by the collection of urine output, and survival was recorded. To ensure reliable pancreatic microcirculation measurement, rats with MAP of < 80 mm Hg, partial pressure of oxygen (pO2) of < 80 mm Hg, pCO2 of > 50 mm Hg, and pH of < 7.3 and dead rats were excluded from the study. At 24 h, laparotomy was performed under ketamine/xylazine anesthesia. The pancreas and spleen were exposed on an adjustable stage. The orthogonal polarization imaging video microscope (Cytoscan A/R, Cytometrics, Philadelphia, PA, USA) was attached to the moveable shaft and the microcirculation was recorded in six different capillary regions of the exocrine pancreas for at least 20 s [17]. Orthogonal polarization imaging was used to measure pancreatic microcirculation [17, 18]. Recorded data were evaluated using an automated microvascular analysis system (MAS, Microvascular Analysis Software, Microvision Medical BV). The FCD was defined as the length of red blood cell-perfused capillaries (in centimeters) per observation area (in square centimeters). Hence we selected FCD as the parameter for measurement of the pancreatic microcirculation. The images were stored in AVI format on a computer (Sony VGN-FW 230J/H).

Figure 1

Experimental design and time schedule of the study

/f/fulltexts/AOMS/29140/AMS-15-29140-g001_min.jpg

Blood samples were taken from the carotid artery for the measurements of serum concentrations of electrolytes, calcium, urea, creatinine, and glucose and activities of amylase, alanine aminotransferase (ALT), and interleukin (IL)-6. A midline sternotomy was performed and the left main bronchus was clamped. Bronchoalveolar lavage (BAL) of the right lung was performed with 2 ml of phosphate-buffered saline containing 0.07 M ethylenediamine tetraacetic acid, and this procedure was repeated twice. The combined lavage of approximately 6 ml was centrifuged at 1,500 rpm for 20 min at 4°C, frozen at –20°C, and assessed subsequently for lactate dehydrogenase (LDH) measurement [20]. The left lung was harvested for the measurements of the activity of the myeloperoxidase (MPO) and malondialdehyde (MDA) levels. The excised lung tissues were rinsed in saline, blotted dry, immediately frozen in liquid nitrogen, and stored at –80°C until MPO and MDA activity assay. At the end of the experiment, the entire pancreas was removed and divided into two samples, one for the histological examination and the other for the measurements of MPO and MDA activity.

In group II (sham + EP, n = 10) animals received EP at 40 mg/kg body weight, given intraperitoneally at the 6th hour. The other procedures were the same as described for the first group. Group III (ANP + saline, n = 16) animals were treated according to the protocol of group 1 after the induction of ANP. In group IV (ANP + EP, n = 16), after induction of ANP, EP was given as described for group 2. Saline and EP were given 6 h after the induction of ANP. In small animals, as ANP occurs four to six times faster than humans and most patients with acute pancreatitis are admitted 24–36 h after the onset of pancreatitis, this period is shorter in order to mimic the clinical situation [21].

Blood pressure monitoring and biochemical analysis

Blood pressure was monitored (Petas KLM 200, Istanbul, Turkey) through a calibrated pressure transducer which was connected to the arterial line. The blood gas analysis were performed using a Ciba Corning 865 analyzer (Chiro Diagnostica Co, East Walpole, MA, USA).

Serum activities of amylase and ALT and the concentrations of glucose, creatinine, urea, calcium, LDH in BAL, and the electrolytes were measured by an autoanalyzer (Vitros 750 autoanalyzer, Johnson & Johnson, Rochester, NY, USA).

Serum IL-6 analysis was performed using enzyme-linked immunosorbent assay (ELISA) (Sanofi Diagnostic Pasteur, Marnes la Coquette, France) with a commercial kit (Biosource Interleukin 6 CB) [22]. The MPO activity was expressed as units per milligram of protein. The MDA measurements were carried out using a colorimetric reaction with thiobarbituric acid by modification of the method described by Buege and Aust [23]. MDA concentrations were expressed as nanomoles per milligram of protein. The protein concentrations of supernatants were measured as described by Lowry [24].

Morphologic examination

Half of the pancreas tissue was stained with hematoxylin-eosin after being fixed in 10% buffered formalin. Histopathological evaluation of each specimen was performed by an experienced pathologist who was blinded to the groups. Necrosis, edema and leukocyte infiltration were classified using a scoring system from 0 to 3 at histopathological investigation. Tissue preparations in which no pathologic changes were observed were scored as 0, while total pancreas necrosis or maximum inflammatory changes were scored as 3 [19].

Statistical analysis

Data are presented as the mean ± SEM. Statistical analyses were performed using SPSS (Statistical Package for the Social Sciences, version 11.5 for Windows, SPSS Inc, Chicago, IL). The difference between the experimental groups with respect to the survival rates was assessed using Fisher’s exact test. Histopathological data and enzyme activities were analyzed using Kruskal-Wallis and Mann-Whitney U tests. A p-value of < 0.05 considered as statistically significant.

Results

Mortality rates for groups were as follows: 0% in the sham + saline group, 43.75% in the ANP + saline group, 0% in the sham + EP group, and 18.75% in the ANP + EP group. Induction of pancreatitis caused significant mortality rates in the pancreatitis groups, while no mortality was observed in the sham groups (p < 0.05; Table I). Mortality was lower in the EP-treated ANP group than the untreated ANP group (44% (7/16) vs. 19% (3/16), respectively, p = 0.05) (Table I).

Table I

Systemic hemodynamic variables, blood gas analysis, mortality and functional capillary density at 24 h (data are given as mean ± SEM)

ParameterSham + saline N = 10Sham + EP N = 10ANP + saline N = 9ANP + EP N = 13
MAP [mm Hg]129 ±8128 ±784 ±4*118 ±8
HR [beat/min]322 ±19329 ±17397 ±18402 ±22
pH7.36 ±0.047.36 ±0.037.3 ±0.047.31 ±0.03
pO2 [mm Hg]108 ±4105 ±482 ±3*98 ±4
pCO2 [mm Hg]34 ±433 ±337 ±338 ±4
Urine [ml/h]0.7 ±0.10.7 ±0.10.3 ±0.1*0.6 ±0.1
Death0/100/107/16**3/16**, *α
FCD [cm/cm²]32 ±333 ±221 ±2*28 ±2

ANP – acute necrotizing pancreatitis, EP – ethyl pyruvate, FCD – functional capillary density, HR – heart rate, MAP – mean arterial pressure.

* p < 0.05, versus other groups

*α p < 0.05 compared with ANP + saline group

** p < 0.005 compared with sham groups.

Administration of EP effectively restored the ANP-induced significant decrease in MAP, and reduction of urine output, pO2 and pancreatic microcirculation (Table I).

Serum activity of amylase, ALT, IL-6, and serum urea was significantly higher and serum calcium concentration was significantly lower (p < 0.05; Table II, Figure 2) following the induction of ANP. The EP treatment provided a significant recovery in these values. No significant change was detected in levels of serum glucose and creatinine in any group (Table II).

Table II

Serum IL-6, tissue concentrations of MPO and MDA, LDH levels in BAL, serum levels of amylase, and the other biochemical parameters at 24 h in animals with or without ANP treated with saline or ethyl pyruvate (data are given as mean ± SEM)

ParameterSham + saline N = 10Sham + EP N = 10ANP + saline N = 9ANP + EP N = 13
Amylase [U/l]1632 ±221570 ±3312300 ±380**9128 ±580**,*α
Glucose [mg/dl]77 ±1581 ±12101 ±8119 ±15
Urea [mg%]18 ±417 ±355 ±7*22 ±3*α
Creatinine [mg%]0.45 ±0.10.44 ±0.10.46 ±0.10.45 ±0.1
ALT [U/l]67 ±769 ±8220 ±17*142 ±12**,*α
Calcium [mg%]8.9 ±0.29.1 ±0.28 ±0.2*8.5 ±0.2*α
BAL LDH [U/l]364 ±22369 ±24790 ±82*313 ±26
IL-6 [pg/ml]870 ±65925 ±565470 ±280**2250 ±180**,*α
Pancreas MDA0.35 ±0.030.33 ±0.031.05 ±0.04*0.5 ±0.06
Pancreas MPO [U/mg total protein]0.26 ±0.010.23 ±0.020.97 ±0.03*0.35 ±0.04
Pancreas MPO [kU/mg MPO protein]2.2 ±0.12.3 ±0.18.2 ±0.3*3.23 ±0.4
Lung MDA0.26 ±0.020.28 ±0.020.90 ±0.04**0.87 ±0.04**
Lung MPO [U/mg total protein]3.3 ±0.033.3 ±0.035.3 ±0.04*3.1 ±0.04
Lung MPO [kU/mg MPO protein]28 ±526 ±448 ±5*33.9 ±6

ALT – alanine aminotransferase, ANP – acute necrotizing pancreatitis, BAL – bronchoalveolar lavage, EP – ethyl pyruvate, LDH – lactate dehydrogenase, MDA – malondialdehyde, MPO – myeloperoxidase.

* p < 0.05 compared with other groups

** p < 0.01 compared with sham groups

*α p < 0.05 compared with ANP + saline group.

Figure 2

Serum IL-6, amylase, and BAL LDH levels among experimental groups (mean ± SEM)

EP – ethyl pyruvate; ANP – acute necrotizing pancreatitis. Asterisks indicate statistically significant difference (*p < 0.05, **p < 0.01 compared with sham groups, θcompared with ANP + saline group).

/f/fulltexts/AOMS/29140/AMS-15-29140-g002_min.jpg

Compared with the sham + saline group the ANP + saline group had significantly higher BAL LDH (364 ±22 U/dl (n = 10) vs. 790 ±82 U/dl (n = 9), p < 0.05), lung MPO (3.3 ±0.3 U/mg protein (n = 10) vs. 5.3 ±0.04 U/mg protein (n = 9), p < 0.05) and lung MDA (0.26 ±0.02 nmol/mg protein (n = 10) vs. 0.90 ±0.04 nmol/mg total protein (n = 11), p < 0.01) (Table II, Figure 3). There were no statistically significant differences in these values between sham + saline and sham + EP groups. When EP was administered (ANP + EP group) there was observed a less pronounced ANP-induced increase in BA LDH and lung MPO activities compared to the ANP + saline group (Table II, Figure 3), but the difference in lung MDA was not significant.

Figure 3

MPO and MDA values in the lung and pancreas tissues among study groups

MPO – myeloperoxidase, MDA – malondialdehyde, EP – ethyl pyruvate, ANP – acute necrotizing pancreatitis. Asterisks indicate statistically significant difference (*p < 0.05, **p < 0.01 compared with sham groups).

/f/fulltexts/AOMS/29140/AMS-15-29140-g003_min.jpg

Histological examination of pancreatic tissue revealed greater edema, necrosis, and leukocyte infiltration in the ANP groups compared to the sham groups (Table III). In the ANP+EP group, the levels of pancreatic edema and necrosis were significantly lower compared to the ANP + saline group (p < 0.05, Table III).

Table III

Histological assessment of edema, necrosis, and inflammation (values are given as mean ± SEM)

ParameterSham + saline N = 10Sham + EP N = 10ANP + saline N = 9ANP + EP N = 13
Edema0.2 ±0.10.2 ±0.20.88 ±0.1**0.5 ±0.2**,*α
Necrosis0.2 ±0.10.2 ±0.21.45 ±0.2**0.96 ±0.2**,*α
Inflammation0.2 ±0.20.2 ±0.21.19 ±0.3**1 ±0.3**

ANP – acute necrotizing pancreatitis, EP – ethyl pyruvate

*p < 0.05 compared with other groups

** p < 0.01 compared with sham groups

*α p < 0.05 compared with ANP + saline group.

Discussion

The results from this experimental study indicate that the induction of ANP caused a significant mortality rate. This was associated with a significant increase in activity of serum amylase, ALT, and IL-6, serum concentration of urea, LDH in BAL fluid, and tissue activity of MPO and MDA in the pancreas and lung. The serum concentrations of calcium, MAP, urine output, pO2 and FCD were significantly decreased. As the main aim of this study was to investigate the effectiveness of EP in experimental ANP, the administration of EP significantly restored these changes.

We utilized the experimental model of ANP as described by Schmidt et al. [19]. Because mild or moderate forms of acute pancreatitis can be self-limiting and be treated with minimal morbidity and mortality, we tested the effects of EP in the severe form of acute pancreatitis [1]. This model provides a superior opportunity to test the effectiveness of novel agents and treatment approaches for ANP by standard processes. It is documented that ANP develops about four to six times faster in small animals than in humans and most acute pancreatitis patients are admitted 24–36 h after its onset [21]. Therefore, we administered EP 6 h after the induction of pancreatitis. It was given as 40 mg/kg body weight, intraperitoneally at 6 h for a single dose. The first step in the treatment of acute pancreatitis is appropriate fluid replacement, so we used appropriate fluid replacement to restore the organ function 6 ml/kg/h after the induction of ANP. Evaluations were limited to the 24-h post-pancreatitis period because the severe form of ANP does not allow long-term survival.

Ethyl pyruvate is a stable simple aliphatic ester with anti-inflammatory actions [14, 25]. Ethyl pyruvate is a comparatively safe and fast acting agent at clinically relevant doses. The only clinical study available demonstrated that 450 mg/kg/day, given in five divided doses, was without side effects [26]. Considering this, the dose used in the current study does not exceed the safe and well-tolerated dose of EP.

It has been shown that EP decreases the release of proinflammatory cytokines such as IL-1β, tumor necrosis factor α (TNF-α), IL-6, and high-mobility group box 1, and also effectively reduces ANP mortality and ameliorates extrapancreatic organ injury in pancreatitis [16, 27, 28]. In severe forms of acute pancreatitis, EP exhibits protective effects by inhibiting activation of nuclear factor-κβ (NF-κβ) [29, 30].

Pancreatic necrosis is the hallmark of severe pancreatitis and is one of the major determinants of mortality [31]. During the course of acute pancreatitis, autodigestion, apoptosis, ischemia, and ischemia-related ROS-associated lipid peroxidation occur and mediate the pancreatic damage [13]. Impairment of microcirculation is another important pathogenetic factor of acute pancreatitis, and evaluation of the microcirculation provides important clues for the status of pancreatitis [3]. The microcirculation of the pancreas can be measured by diffuse reflectance spectroscopy, multiple indicator dilution technique, intravital microscopy, and orthogonal polarization imaging [21]. In the current study, pancreatic microcirculation was measured by orthogonal polarization imaging technique. Oxidative stress and ROS are important mediators in the pathogenesis of acute pancreatitis [7, 32]. In our study, induction of pancreatitis resulted in a decrease in FCD and increase in ischemic mediators such as pancreatic tissue MPO and MDA activities, which reflect oxidative stress. Administration of EP restored the decreased FCD value and increased pancreatic tissue ischemia mediators. The obtained improvement by EP treatment in our model may be due to antioxidative effects of EP. Recent evidence indicates that EP also has anticoagulant properties, and the obtained improvement in pancreatic microcirculation may also involve the anticoagulant effects of EP [3335].

In the early stage of severe pancreatitis acute lung injury and acute respiratory distress syndrome (ARDS) often develop and have been considered among the major causes of early death [36, 37]. The production of ROS by the activated leukocytes resulting in increased capillary permeability plays a major role in the pathogenesis of ARDS. Therefore, BAL fluid LDH levels were used for capillary permeability assessment [20, 37]. The induction of ANP resulted in increased LDH in BAL fluid, decreased pO2, and increased activities of MPO and MDA in lung tissue. Administration of EP significantly reduced the lung injury associated with the pancreatitis. Matone et al. tested EP and reported similar results to our study in rat lung [38].

In acute pancreatitis, direct damage to pancreatic tissue as a result of the systemic inflammatory response and activation of various enzymes and cytokines often leads to multiorgan failure. Administration of EP provided significant improvements in the pancreatitis-induced changes in the concentration of serum urea and ALT activity and serum calcium and urine output. NF-κβ activation has an important role in the progression from the localized disease to multiorgan failure in acute pancreatitis. The inhibitory effect of EP on activation of NF-κβ may explain its protective effects on other organs [27, 28].

It has been shown that measurement of activation of proteolytic peptides (e.g. trypsinogen-activated peptide) and activity level of acute-phase reactant and markers of inflammation including C-reactive protein, TNF-α, IL-6, and IL-10 reliably reflect the severity and outcome of acute pancreatitis [39]. In the current study serum IL-6 was measured as a marker of inflammation and a significant improvement in serum IL-6 levels was obtained with the use of EP. The EP has an inhibitory effect on some of the pro-inflammatory cytokines through the inhibition of NF-κβ, and these may have mediated the observed beneficial effects of EP on ANP, as reflected by pancreatic tissue damage and the overall mortality rate [27, 28, 38].

This study has some limitations that need to be considered when interpreting data. This is an animal study employing experimental ANP, and the translational potential needs to be confirmed by clinical studies. We demonstrated a beneficial effect of EP without clarifying the exact mechanism of action. Although there are definitely anti-oxidant effects of EP, the protective effects of this agent may extend beyond the anti-oxidant influences and include anti-inflammatory actions. We used a single dose and single administration of EP; considering the obtained beneficial effect of chronic application, higher doses are warranted.

In conclusion, we examined the effect of EP, a novel endogenous metabolite with prominent anti-inflammatory and antioxidant activity, in a severe form of experimental ANP and found that EP effectively reduces mortality and pancreatic damage during acute pancreatitis. Thus, positive effects of EP on the course of ANP in rats may have important implications, indicating that EP is a promising molecule that could be used either alone or in combination with other substances for the management of ANP.

Acknowledgments

This research was supported by a grant from Karadeniz Technical University, Scientific Research Projects Unit (under grant number 2008.114.002.14).

Conflict of interest

The authors declare no conflict of interest.

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