eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank

vol. 40
Experimental immunology

Evaluation of anti-inflammatory and analgesic activities of extracts from herb of Chelidonium majus L.

Przemysław Łukasz Mikołajczak
Bogdan Kędzia
Marcin Ożarowski
Radosław Kujawski
Anna Bogacz
Joanna Bartkowiak-Wieczorek
Wojciech Białas
Agnieszka Gryszczyńska
Waldemar Buchwald
Michał Szulc
Natalia Wasiak
Małgorzata Górska-Paukszta
Justyna Baraniak
Bogusław Czerny
Agnieszka Seremak-Mrozikiewicz

(Cent Eur J Immunol 2015; 40 (4): 400-410)
Online publish date: 2016/01/15
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
The aim of the study was to evaluate analgesic activity (“hot plate” test), anti-inflammatory activity (carrageenan-induced paw edema) and locomotor activity in rats under the influence of three fractions of Chelidonium majus herb extract: full water extract (FWE), protein enriched fraction (PEF), and non-protein fraction (NPF). Effects of the fractions on the level of chosen cytokines and their mRNA levels were also assessed using lipopolysaccharide (LPS) administration as a proinflammatory cue. All fractions and diclofenac did not affect the locomotor activity of rats in comparison with the control group. FWE and PEF three hours after administration showed statistically significant analgesic activities comparable to morphine (p < 0.05). A slight reduction in rat paw edema was observed after three (comparable with diclofenac) and six hours in the NPF group. FWE revealed a statistically significant pro-inflammatory effect after three hours in comparison with the control group. Peripheral IL-1 and IL-4 cytokine concentrations were reduced under FWE and NPF, PEF fractions. The combination of FWE, PEF and NPF together with LPS showed only the effects of LPS. We suggest that protein enriched fraction (PEF) produced centrally mediated (morphine-like) analgesic action, whereas the anti-inflammatory potential was shown only after LPS-induced inflammation. The precise mechanisms involved in the production of anti-nociceptive and anti-inflammatory responses of studied fractions are not completely understood, but they may be caused rather by the presence of protein more than alkaloids-enriched fraction. This fraction of the extract could be used as an alternative therapy for the prevention of inflammatory-related diseases in the future, but further studies are needed.

LPS–induced rats, analgesic, anti-inflammatory, Chelidonium majus aqueous extract, non-protein fraction, protein enriched fraction, proinflammatory cytokines, mRNA

Quick links
© 2020 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe