eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank
2/2013
vol. 38
 
Share:
Share:
more
 
 
abstract:

Experimental immunology
Assessment of the potential genotoxic and proapoptotic impact of selected cyanotoxins on fish leukocytes

Anna Sierosławska
,
Anna Rymuszka

(Centr Eur J Immunol 2013; 38 (2): 190-195)
Online publish date: 2013/07/08
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Cyanotoxins, toxic secondary metabolites of cyanobacteria, are able to induce a wide range of negative health effects in the intoxicated organisms. Except for their well-described hepatotoxic or neurotoxic activities, cyanotoxins are also considered to possess other properties, such as immunotoxicity or genotoxicity. The aim of the work was to study the potential cytotoxic/genotoxic impact of selected cyanotoxins (microcystin-LR, cylindrospermopsin and anatoxin-a) on common carp leukocytes. The cells were exposed to the toxins at 0.5 µg/ml for 18 h. DNA damage in the cells was studied with the use of the alkaline version of the comet assay detecting single- and double-strand DNA breaks. Apoptotic cells were identified on the basis of phosphatidylserine translocation in the plasma membrane and the cell viability was checked by the propidium iodide staining. The most substantial changes were detected in the leukocytes exposed to microcystin-LR, in which a high level of DNA damage was observed, but there was also a high number of apoptotic cells. No such effects were observed after cell exposure to anatoxin-a. Cylindrospermopsin did not intensify apoptosis or necrosis, however some increase in DNA damage was detected.
keywords:

microcystin-LR, cylindrospermopsin, anatoxin-A, carp, genotoxicity, apoptosis, necrosis

Quick links
© 2022 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.