Experimental immunology<br>Cloning and analysis of Nile tilapia Toll-like receptors type-3 mRNA

Reham R. Abouelmaatti; Abdelazeem M. Algammal; Xiaokun Li; Jisheng Ma; Eman A. Abdelnaby; Wael M.K. Elfeil

doi:10.5114/ceji.2013.37740

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2013
vol. 38

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abstract:

Experimental immunology
Cloning and analysis of Nile tilapia Toll-like receptors type-3 mRNA

Reham R. Abouelmaatti

,

Abdelazeem M. Algammal

,

Xiaokun Li

,

Jisheng Ma

,

Eman A. Abdelnaby

,

Wael M.K. Elfeil

(Centr Eur J Immunol 2013; 38 (3): 277-282)

DOI: https://doi.org/10.5114/ceji.2013.37740

Online publish date: 2013/10/28

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Toll-like receptors (TLRs) are the best understood of the innate immune receptors that detect infections in vertebrates. However, the fish TLRs also exhibit very distinct features and a large diversity, which is likely derived from their diverse evolutionary history and the distinct environments that they occupy. Little is known about the fish immune system structure. Our work was aimed to identify and clone the Nile tilapia TLR-3 as a model of fresh water fish species; we cloned the full-length cDNA sequence of Nile tilapia (Oreochromis niloticus) TLR-3 and according to our knowledge, it is the first report illustrating tilapia TLR-3.

The complete cDNA sequence of Nile tilapia TLR-3 was 2736 pair base and it encodes a polypeptide of 912 amino acids. Analysis of the deduced amino acid sequence indicated that Nile tilapia TLR-3 has typical structural features and main component of proteins belonging to the TLR family. Our results illustrate a complete and functional Nile tilapia TLR-3 and it is considered an ortholog of the other vertebrate’s receptor.

keywords:

Experimental immunologyCloning and analysis of Nile tilapia Toll-like receptors type-3 mRNA

Reham R. Abouelmaatti , Abdelazeem M. Algammal , Xiaokun Li , Jisheng Ma , Eman A. Abdelnaby , Wael M.K. Elfeil

Nile tilapia, single nucleotide polymorphisms, fish, Toll-like receptor 3 – TLR-3, gene expression

Experimental immunology
Cloning and analysis of Nile tilapia Toll-like receptors type-3 mRNA

Reham R. Abouelmaatti

,

Abdelazeem M. Algammal

,

Xiaokun Li

,

Jisheng Ma

,

Eman A. Abdelnaby

,

Wael M.K. Elfeil