Experimental immunology<br>Potential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation

Ping Yang; Yilu Lu; Xue Jiang; Minhui Li; Chao Li; Huijuan Chen; Kun Zhang; Kejian Pan; Dachang Tao; Sizhong Zhang; Yongxin Ma

doi:10.5114/ceji.2013.34353

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Experimental immunology
Potential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation

Ping Yang

,

Yilu Lu

,

Xue Jiang

,

Minhui Li

,

Chao Li

,

Huijuan Chen

,

Kun Zhang

,

Kejian Pan

,

Dachang Tao

,

Sizhong Zhang

,

Yongxin Ma

(Centr Eur J Immunol 2013; 38 (1): 15-22)

DOI: https://doi.org/10.5114/ceji.2013.34353

Online publish date: 2013/04/17

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AMA

Yang P, Lu Y, Jiang X, et al. Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation. Central European Journal of Immunology. 2013;38(1):15-22. doi:10.5114/ceji.2013.34353.

APA

Yang, P., Lu, Y., Jiang, X., Li, M., Li, C.,  & Chen, H. et al. (2013). Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation. Central European Journal of Immunology, 38(1), 15-22. https://doi.org/10.5114/ceji.2013.34353

Chicago

Yang, Ping, Yilu Lu, Xue Jiang, Minhui Li, Chao Li, Huijuan Chen,  and Kun Zhang et al. 2013. "Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation". Central European Journal of Immunology 38 (1): 15-22. doi:10.5114/ceji.2013.34353.

Harvard

Yang, P., Lu, Y., Jiang, X., Li, M., Li, C., Chen, H., Zhang, K., Pan, K., Tao, D., Zhang, S.,  and Ma, Y. (2013). Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation. Central European Journal of Immunology, 38(1), pp.15-22. https://doi.org/10.5114/ceji.2013.34353

MLA

Yang, Ping et al. "Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation." Central European Journal of Immunology, vol. 38, no. 1, 2013, pp. 15-22. doi:10.5114/ceji.2013.34353.

Vancouver

Yang P, Lu Y, Jiang X, Li M, Li C, Chen H et al. Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation. Central European Journal of Immunology. 2013;38(1):15-22. doi:10.5114/ceji.2013.34353.

PlumX metrics:

RING (really interesting new gene) finger protein 166, or RNF166, belongs to a C3HC4 ubiquitin ligases subfamily, which include four related proteins containing a conserved C3HC4 (Cys3-His-Cys4) RING finger domain. RNF125, one member of the subfamily, has been identified as a regulator of T cell activation, but the potential roles of another member RNF166 remains poorly understood. Here we reported that RNF166 is involved in regulation of T cell activation. Flow cytometry (FCM) data showed that overexpression of RNF166 in primary T cells and Jurkat T cells induced over 2-fold increase of CD69, a T-cell activation marker, suggesting that RNF166 is a positive-regulator of T cell activation. Furthermore, pull-down assays showed that RNF166 can bind with both Lys⁴⁸-linked polyubiquitin and Lys⁶³-linked polyubiquitin, indicating that RNF166 may play regulating roles in T cell activation by self-degradation via ubiquitin-proteasome pathway and/or cross-talk with certain signaling pathways via non-proteasome-dependent pathways. In conclusion, our work reveals that RNF166 is a potential positive-regulator of T cell activation and these findings provide a novel insight into understanding the functions of RNF166 in the positive regulation of immune responses.

keywords:

Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation

Ping Yang , Yilu Lu , Xue Jiang , Minhui Li , Chao Li , Huijuan Chen , Kun Zhang , Kejian Pan , Dachang Tao , Sizhong Zhang , Yongxin Ma

flow cytometry, pull down, regulator, RING finger protein 166, T cell activation

Experimental immunology
Potential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation

Ping Yang

,

Yilu Lu

,

Xue Jiang

,

Minhui Li

,

Chao Li

,

Huijuan Chen

,

Kun Zhang

,

Kejian Pan

,

Dachang Tao

,

Sizhong Zhang

,

Yongxin Ma