Experimental immunology<br>Proteomic and immunological identification of two new allergens from silkworm (<i>Bombyx mori </i>L.) pupae

Xiangjie Zhao; Lin Li; Zheshi Kuang; Guoqing Luo; Bing Li

doi:10.5114/ceji.2015.50830

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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1/2015
vol. 40

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abstract:

Experimental immunology
Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae

Xiangjie Zhao

,

Lin Li

,

Zheshi Kuang

,

Guoqing Luo

,

Bing Li

(Centr Eur J Immunol 2015; 40 (1): 30-34)

DOI: https://doi.org/10.5114/ceji.2015.50830

Online publish date: 2015/04/22

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This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin E (IgE)-binding proteins by western blotting, and allergens specific for silkworm pupa consumption-caused allergy were visualised with the ECL reagents. The selected allergen proteins were further identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding reaction. Analysis of the sequence homology of the two proteins using the AllergenOnline database indicated that chitinase and paramyosin shared 24.8% and 62.8% sequence homology with known allergens Der f 18 (Dermatophagoides farinae) and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed light on the understanding and treatment of silkworm pupa allergy.

keywords:

Experimental immunologyProteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae

Xiangjie Zhao , Lin Li , Zheshi Kuang , Guoqing Luo , Bing Li

silkworm (Bombyx mori L) pupa, allergen, chitinase, paramyosin, western blot, MALDI-TOF-MS

Experimental immunology
Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae

Xiangjie Zhao

,

Lin Li

,

Zheshi Kuang

,

Guoqing Luo

,

Bing Li