eISSN: 2449-8580
ISSN: 1734-3402
Family Medicine & Primary Care Review
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank

 
1/2021
vol. 23
 
Share:
Share:
more
 
 
abstract:
Original paper

Expression of inflammatory markers and NMDA receptors in the peripheral blood of stroke patients

Abolfazl Azami Tameh
1
,
Bahareh Bahmani
1, 2
,
Mohammad Karimian
3
,
Ebrahim Kouchaki
1
,
Hamidreza Talari
1
,
Cordian Beyer
4

1.
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
2.
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
3.
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
4.
Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Aachen, Germany
Family Medicine & Primary Care Review 2021; 23(1): 75–80
Online publish date: 2021/04/02
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Background
Identifying early molecular markers in ischemic stroke could help in reducing acute adverse effects of brain tissue after recanalization of clogged blood vessels.

Objectives
In this study, we evaluated the gene expression levels of glutamate N-methyl-D-aspartate receptor (NMDAR) subunits (NR2A and NR3A) and inflammatory markers, as well as some oxidative stress markers in the peripheral blood of ischemic patients 24 h and 72 h after admission to the hospital.

Material and methods
38 patients with ischemic stroke and 29 non-stroke individuals were included in the study. Prognosis and long-term outcomes of patients were evaluated using infarct area measurement from computed tomography scans and the Scandinavian neurological stroke scale. To identify serum biomarker changes, malondialdehyde (MDA) and NO (nitric oxide) levels were measured. A quantitative real-time PCR protocol was performed in order to evaluate the gene expression changes of NR2A, NR3A, TNF-α and interleukin-6 in selectively-isolated peripheral lymphocyte.

Results
Ischemic stroke induced a massive loss of lymphocytes (as determined by total lymphocyte RNA levels). The mRNA levels of NR2A and NR3A were also significantly decreased 24 h and 72 h after admission to the hospital. We also observed a down-regulation of TNF-α and IL-6 gene expression during the first day after admission. The serum level of NO showed a reduction from day one to day three, while MDA increased on day one of admission.

Conclusions
Changes in inflammatory cytokines following an ischemic attack could stimulate glutamate receptors in peripheral lymphocytes. As preliminary findings, this phenomenon could be considered as a prognostic and therapeutic tool in ischemic stroke management.

keywords:

ischemic stroke, NMDA receptor, peripheral blood, lymphocyte, inflammation

 
Quick links
© 2021 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe