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Archives of Medical Science
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vol. 14
Clinical research

Glycoprotein Ibα Kozak polymorphism in patients presenting with early-onset acute coronary syndrome

Ebru Golcuk, Kivanc Yalin, Cansu Selcan Akdeniz, Erhan Teker, Başak Teker, Veysel Sabri Hancer, Ibrahim Altun, Murat Sezer, Reyhan Diz Kucukkaya, Aytac Oncul

Arch Med Sci 2018; 14, 4: 788–793
Online publish date: 2016/10/26
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Glycoprotein Ibα (GPIbα) receptor is the chief molecule responsible for initial platelet adhesion to the subendothelium. A thymidine to cytosine single nucleotide substitution at position –5 from the ATG start codon characterizes the Kozak sequence polymorphism. The Kozak sequence polymorphism may increase the surface expression of GPIbα and contribute to thrombogenesis. We evaluated the allele frequencies of GPIbα Kozak sequence polymorphism in the Turkish population and examined the relationship between GPIbα Kozak sequence polymorphism and early-onset acute coronary syndrome (ACS).

Material and methods
This study enrolled 200 patients (122 male, 78 female, mean age: 39 ±5 years) and 200 healthy control subjects (110 male, 90 female, 41 ±4 years). The patient group was composed of patients admitted to our coronary care unit with early-onset ACS and patients who attended to our cardiology outpatient clinic after hospital discharge with a diagnosis of early-onset ACS.

Kozak polymorphism frequencies in patients and control subjects did not differ significantly (23% versus 22.5%, p = 0.812, respectively). In patients who presented with non-ST elevation myocardial infarction (NSTEMI), the frequency of GPIbα Kozak polymorphism was borderline significantly higher when compared with patients who presented with ST elevation myocardial infarction (STEMI) (35% vs. 20%, p = 0.05, respectively). Allele frequencies of T and C were calculated to be 0.873 and 0.128.

Although the frequency of GPIbα Kozak polymorphism did not differ significantly in early-onset ACS patients versus control subjects, Kozak polymorphism frequency was borderline significantly higher in patients who presented with NSTEMI when compared to patients with STEMI.


Turkish population, acute coronary syndrome, glycoprotein Ibα Kozak polymorphism

Napoleao P, Morteiro Mdo C, Cabral LB, et al. Changes of soluble CD40 ligand in the progression of acute myocardial infarction associate to endothelial nitric oxide synthase polymorphisms and vascular endothelial growth factor but not to platelet CD62P expression. Transl Res 2015; 166: 650-9.
Shen GQ, Girelli D, Li L, et al. Multi-allelic haplotype association identifies novel information different from single-SNP analysis: a new protective haplotype in the LRP8 gene is against and early-onset CAD and MI. Gene 2013; 521: 78-81.
Shen GQ, Girelli D, Li L, et al. A novel molecular diagnostic marker for familial and early-onset coronary artery disease and myocardial infarction in the LRP8 gene. Circ Cardiovasc Genet 2014; 7: 514-20.
Hartopo AB, Puspitawati I, Gharini PPR, et al. Platelet microparticle number is associated with the extent of myocardial damage in acute myocardial infarction. Arch Med Sci 2016; 12: 529-37.
Meisel C, Afshar-Kharghan V, Cascorbi I, et al. Role of Kozak sequence polymorphism of platelet glycoprotein Ibalpha as a risk factor for coronary artery disease and catheter interventions. J Am Coll Cardiol 2001; 38: 1023-7.
Kenny D, Muckian C, Fitzgerald DJ, Cannon CP, Shields DC. Platelet glycoprotein Ib alpha receptor polymorphisms and recurrent ischaemic events in acute coronary syndrome patients. J Thromb Thrombolysis 2002; 13: 13-9.
Douglas H, Michaelides K, Gorog DA, et al. Platelet membrane glycoprotein Ibalpha gene -5T/C Kozak sequence polymorphism as an independent risk factor for the occurrence of coronary thrombosis. Heart 2002; 87: 70-4.
Pellikka M, Narhi L, Perola M, Penttila A, Karhunen PJ, Mikkelsson J. Platelet GPIbalpha, GPIV and vWF polymorphisms and fatal pre-hospital MI among middle-aged men. J Thromb Thrombolysis 2008; 26: 91-6.
Esen FI, Hancer VS, Kucukkaya RD, et al. Glycoprotein Ib-alpha Kozak polymorphism in ischemic stroke. Neurol Res 2012; 34: 68-71.
Song YQ, Zou HL, Zhang C. Correlation between the polymorphism of platelet glycoprotein GPIb alpha Kozak gene and transient ischemic attack. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2006; 23: 561-4.
Hsieh K, Funk M, Schillinger M, et al. Vienna Stroke Registry. Impact of the platelet glycoprotein Ib alpha Kozak polymorphism on the risk of ischemic cerebrovascular events: a case-control study. Blood Coagul Fibrinolysis 2004; 15: 469-73.
Frank MB, Reiner AP, Schwartz SM, et al. The Kozak sequence polymorphism of platelet glycoprotein Ibalpha and risk of nonfatal myocardial infarction and nonfatal stroke in young women. Blood 2001; 97: 875-9.
Ozelo MC, Origa AF, Aranha FJ, et al. Platelet glycoprotein Ibalpha polymorphism modulate the risk for myocardial infarction. Thromb Haemost 2004; 92: 384-6.
Croft SA, Hampton KK, Daly ME, Steeds RP, Channer KS, Samani NJ. Kozak sequence polymorhism in the platelet GPIbalpha gene is not associated with risk of myocardial infarction. Blood 2000; 95: 2183-4.
Candore G, Piazza G, Crivello A, et al. Association between platelet glycoprotein Ib-alpha and myocardial infarction: results of a pilot study performed in male and female patients from Sicily. Ann N Y Acad Sci 2006; 1089: 502-8.
Ozelo MC, Origa AF, Aranha FJ, et al. Platelet glycoprotein Ibalpha polymorphisms modulate the risk for myocardial infarction. Thromb Haemost 2004; 92: 384-6.
Thygesen K, Alpert JS, Jaffe AS, Simoons ML, Chaitman BR, White HD; the Writing Group on behalf of the Joint ESC/ACCF/AHA/WHF Task Force for the Universal Definition of Myocardial Infarction. Third universal definition of myocardial infarction. Eur Heart J 2012; 33: 2551-67.
Afshar-Kharghan V, Li CQ, Khoshnevis-Asl M, Lopez JA. Kozak sequence polymorphism of the glycoprotein (GP) Ibalpha gene is a major determinant of the plasma membrane levels of the platelet GPIb-IX-V complex. Blood 2000; 95: 2183-4.
Huang T, Sahud MA. Association of C807T, Pl(A), and -5 C/T Kozak genotypes with density of glycoprotein receptors on platelet surface. Thromb Res 2003; 112: 147-50.
Bondarenko EA, Shetova IM, Shamalov NA, et al. Analysis association of acute ischemic stroke and DNA markers in Russian and Moldavian populations. Genetika 2011; 47: 1393-401.
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