Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and <i>in vitro</i> functional studies

Anna Mania; Mariusz Kaczmarek; Iwona Mozer-Lisewska; Arleta Kowala-Piaskowska; Agata Kolecka-Bednarczyk; Magdalena Frydrychowicz; Bartosz Brzezicha; Rifaat Safadi; Anna Pauli; Husam Samara; Jan Żeromski

doi:10.5114/ceji.2013.39766

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

**Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies**

Anna Mania

,

Mariusz Kaczmarek

,

Iwona Mozer-Lisewska

,

Arleta Kowala-Piaskowska

,

Agata Kolecka-Bednarczyk

,

Magdalena Frydrychowicz

,

Bartosz Brzezicha

,

Rifaat Safadi

,

Anna Pauli

,

Husam Samara

,

Jan Żeromski

(Centr Eur J Immunol 2013; 38 (4): 486-493)

DOI: https://doi.org/10.5114/ceji.2013.39766

Online publish date: 2013/12/30

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AMA

Mania A, Kaczmarek M, Mozer-Lisewska I, et al. Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies. Central European Journal of Immunology. 2013;38(4):486-493. doi:10.5114/ceji.2013.39766.

APA

Mania, A., Kaczmarek, M., Mozer-Lisewska, I., Kowala-Piaskowska, A., Kolecka-Bednarczyk, A.,  & Frydrychowicz, M. et al. (2013). Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies. Central European Journal of Immunology, 38(4), 486-493. https://doi.org/10.5114/ceji.2013.39766

Chicago

Mania, Anna, Mariusz Kaczmarek, Iwona Mozer-Lisewska, Arleta Kowala-Piaskowska, Agata Kolecka-Bednarczyk, Magdalena Frydrychowicz,  and Bartosz Brzezicha et al. 2013. "Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies". Central European Journal of Immunology 38 (4): 486-493. doi:10.5114/ceji.2013.39766.

Harvard

Mania, A., Kaczmarek, M., Mozer-Lisewska, I., Kowala-Piaskowska, A., Kolecka-Bednarczyk, A., Frydrychowicz, M., Brzezicha, B., Safadi, R., Pauli, A., Samara, H.,  and Żeromski, J. (2013). Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies. Central European Journal of Immunology, 38(4), pp.486-493. https://doi.org/10.5114/ceji.2013.39766

MLA

Mania, Anna et al. "Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies." Central European Journal of Immunology, vol. 38, no. 4, 2013, pp. 486-493. doi:10.5114/ceji.2013.39766.

Vancouver

Mania A, Kaczmarek M, Mozer-Lisewska I, Kowala-Piaskowska A, Kolecka-Bednarczyk A, Frydrychowicz M et al. Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies. Central European Journal of Immunology. 2013;38(4):486-493. doi:10.5114/ceji.2013.39766.

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Background: HSC constitute a small portion of liver cells, but fulfill significant functions. LX-2 cells, a human cell line of HSC, are a model for the study of HSC. HSC express Toll-like receptors (TLRs) and some of them participate in the promotion of liver fibrosis. Little is known about other TLRs expression and function of HSC. Aims of this study were to search the expression and function of all TLRs known in LX-2 cells.

Material and methods: Cells were subjected to immunocytochemistry, cell RNA was searched for mRNA specific for respective TLRs. Cells were treated with anti-TLR1-10 antibodies and flow cytometry (FC). They were cultured with TLR ligands and assessed by FC for TLR expression. Supernatants were examined for cytokines content and cells for the percentage of those in a S-phase of cell cycle.

Results: All TLRs, both, protein and mRNA were expressed in these cells. TLR ligands had negligible effect on TLR expression. Culture of LX-2 with ligands resulted in the secretion of some cytokines, but did not increase the number of cells in S-phase.

Conclusions: To conclude, LX-2 cells express all known TLRs. Their activation has only modest effect on their function.

keywords:

Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies

Anna Mania , Mariusz Kaczmarek , Iwona Mozer-Lisewska , Arleta Kowala-Piaskowska , Agata Kolecka-Bednarczyk , Magdalena Frydrychowicz , Bartosz Brzezicha , Rifaat Safadi , Anna Pauli , Husam Samara , Jan Żeromski

hepatic stellate cells, LX-2 cells, Toll-like receptors, TLR function

**Hepatic stellate cells (HSC) cell line (LX-2) and Toll-like receptors – phenotypic/mRNA expression and in vitro functional studies**

Anna Mania

,

Mariusz Kaczmarek

,

Iwona Mozer-Lisewska

,

Arleta Kowala-Piaskowska

,

Agata Kolecka-Bednarczyk

,

Magdalena Frydrychowicz

,

Bartosz Brzezicha

,

Rifaat Safadi

,

Anna Pauli

,

Husam Samara

,

Jan Żeromski