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Archives of Medical Science
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5/2018
vol. 14
 
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abstract:
Experimental research

Hypoglycaemic activity of 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione in streptozotocin-induced diabetic mice through ameliorating metabolic function and regulating peroxisome proliferator-activated receptor γ

Kintoko Kintoko, Xiaohui Xu, Xing Lin, Yang Jiao, Qingwei Wen, Zhaoni Chen, Jinbin Wei, Tao Liang, Renbin Huang

Arch Med Sci 2018; 14, 5: 1163–1172
Online publish date: 2016/10/26
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Introduction
Diabetes mellitus is characterized by hyperglycaemia causing changes in plasma lipoproteins, which leads to insulin resistance, secretion defects or both. The present study aimed to evaluate the ability of 2-dodecyl-6-methoxy-cyclohexa-2,5-diene-1,4-dione (DMDD) isolated from Averrhoa carambola L. roots to lower hyperglycaemia and to investigate its potential mechanism in diabetic mice.

Material and methods
DMDD was isolated using a column chromatographic technique. Experimental mice were fed with a high-fat diet for a month and were intravenously injected with streptozotocin (80 mg/kg, single dose). Diabetic mice were orally administered DMDD (12.5, 25, 50 mg/kg) and 50 mg/kg pioglitazone for 15 days. Fasting blood glucose (FBG), fasting blood insulin (FINS), pancreatic insulin content, interleukin-6 (IL-6), tumour necrosis factor- (TNF-), as well as serum total cholesterol (TC), triglyceride (TG) and free fatty acid (FFA) were determined. Adipose tissue was assessed by histological examination, immunohistochemistry, western blot and reverse transcription-polymerase chain reaction methods.

Results
DMDD significantly increased the insulin level (all p < 0.05). In contrast, FBG, IL-6, TNF-, TC, TG and FFA were significantly decreased (all p < 0.05). However, DMDD induced the activation of adipocyte peroxisome proliferator-activated receptor  (PPAR-), confirmed by increased protein and mRNA expression of PPAR-

Conclusions
DMDD possessed hypoglycaemic activity due to its potential mechanism involving PPAR-mediated adipocyte endocrine regulation.

keywords:

Averrhoa carambola L, diabetes, mechanism, adipocyte, endocrine, peroxisome proliferator-activated receptor γ

references:
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2006; 29 (suppl 1): S43-8.
Zimmet P. The burden of type 2 diabetes: are we doing enough? Diabetes Metab 2003; 29: 6S9-18.
International Diabetes Federation. Type 1 diabetes: a very special issue. Diabetes Voice 2011; 56: 4-49.
Sun K, Li F, Qi Y, et al. Sex difference in the association between habitual daytime napping and prevalence of diabetes: a population-based study. Endocrine 2016; 52: 263-70.
Wong KC, Wang Z. Prevalence of type 2 diabetes mellitus of Chinese populations in mainland China, Hongkong, and Taiwan. Diabetes Res Clin Pract 2006; 73: 126-34.
Dembitsky VM, Poovarodom S, Leontowicz H, et al. The mutiple nutrition properties of some exotic fruits: biological activity and active metabolites. Food Res Int 2011; 44: 1671-701.
Gunasekara LCA, Fernando PHP, Sivakanesan R. A preliminary study on the hypoglycaemic effect of Averrhoacarambola (star fruit) in rats. Proceedings of the Peradeniya University Research Sessions, Sri Lanka 2011.
Chau CF, Chen CH, Lee MH. Characterization and physiochemical properties of some potential fibers derived from Averrhoacarambola. Nahrung 2004; 48: 43-6.
Ferreira EB, Fernandes LC, Galende SB, Cortez DAG, Bazotte RB. Hypoglycemic effect of the hydroalcoholic extract of leaves of Averrhoacarambola L. (Oxalidaceae). Brazilian J Pharmacog 2008; 18: 339-43.
Cazarolli LH, Kappel VD, Pereira DF, et al. Anti-hyperglycaemic action of apigenin-6-C-beta-fucopyranoside from Averrhoacarambola. Fitoterapia 2012; 83: 1176-83.
Cazarolli LH, Folador P, Moresco HH, Brighente IM, Pizzolatti MG, Silva FR. Mechanism of action of the stimulatory effect of apigenin-6-C-beta-fucopyranoside on 14C-glucose uptake. Chem Biol Interact 2009; 179: 407-12.
Zheng N, Lin X, Wen Q, et al. Effect of 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione, isolated from Averrho-acarambola L. (Oxalidaceae) roots, on advanced glycation end-product-mediated renal injury in type 2 diabetic KKAy mice. Toxicol Lett 2013; 29: 77-84.
Ivorra MD, Paya M, Villar A. A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol 1989; 27: 243-75.
Dandawate PR, Vyas AC, Padhye SB, Singh MW, Baruah JB. Perspectives on medicinal properties of benzoquinone compounds. Mini Rev Med Chem 2010; 10: 436-54.
Gupta R, Sharma AK, Sharma MS, Gupta RS. Antioxidant activity and protection of pancreatic beta-cells by embelin in streptozotocin-induced diabetes. J Diabetes 2012; 4: 248-56.
Wen Q, Lin X, Liu Y, et al. Phenolic and lignan glycosides from the butanol extract of Averrhoa carambola L. root. Molecules 2012; 17: 12330-40.
Ginsberg HN, Zhang YL, Hernandez-Ono A. Regulation of plasma triglyceride in insulin resistance and diabetes. Arch Med Res 2005; 36: 232-40.
van den Oever IA, Raterman HG, Nurmohamed MT, Simsek S. Endothelial dysfunction, inflammation, and apoptosis in diabetes mellitus. Mediators Inflamm 2010; 2010: 792393.
Rangwala SM, Lazar MA. Peroxisome proliferator-activated receptor in diabetes and metabolism. Trends Pharmacol Sci 2004; 25: 331-6.
Higa M, Zhou, YT, Ravazzola M, Baetens D, Orci L, Unger RH. Troglitazone prevents mitochondrial alterations, beta cell destruction, and diabetes in obese prediabetic rats. Proc Natl Acad Sci USA 1996; 96: 11513-8.
Xu X, Liang T, Lin X, et al. Effect of the total extract of Averrhoacarambola (Oxalidaceae) root on the expression levels of TLR4 and NF-kappaB in streptozotocin-induced diabetic mice. Cell Physiol Biochem 2015; 36: 2307-16.
Naik SR, Niteru NT, Ansari AA, Shah PD. Anti-diabetic activity of embelin: involevement of cellular inflammatory mediators, oxidative stress and other biomarkers. Phytomedicine 2013; 20: 797-804.
Bhandari U, Jain N, Ansari MN, Pillai KK. Beneficial effect of Embeliaribesethanolic extract on blood ressure and glycosylated haemoglobin in streptozotocin-induced diabetes in rats. Fitoterapia 2008; 79: 351-5.
Mahendran S, Badami S, Maithili V. Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats. Biomed Prevent Nutr 2011; 1: 25-31.
Marchetti P, Dotta F, Lauro D, Purrello F. An overview of pancreatic beta-cell defects in human type 2 diabetes: implication for treatment. Regular Pept 2008; 146: 4-11.
United Kingdom Prospective Diabetes Study Group 16. Overview of 6 years’ therapy of type 2 diabetes: a progressive disease. Diabetes 1995; 44: 1249e58.
Desgraz R, Bonal C, Herrera PL. Beta-cell regeneration: the pancreatic intrinsic faculty. Trends Endocrin Metabol 2011; 22: 34-43.
Berhanu P, Kipnes MS, Khan MA, et al. Effects of pioglitazone on lipid and lipoprotein profiles in patients with type 2 diabetes and dyslipidaemia after treatment conversion from rosiglitazone while continuing stable statin therapy. Diabet Vascul Dis Res 2006; 3: 39-44.
Lewis GF. Fatty acid regulation of very low density lipoprotein (VLDL) production. Curr Opin Lipidol 1999; 10: 475-7.
Katsiki N, Nikolic D, Montalto G, Banach M, Mikhailidis DP, Rizzo M. The role of fibrate treatment in dyslipidemia: an overview. Curr Pharm Des 2013; 19: 3124-31.
Banach M, Aronow WS, Serban MC, Rysz J, Voroneanu L, Covic A. Lipids, blood pressure and kidney update 2015. Lipids Health Dis 2015; 14: 167.
Liu A, Sonmez A, Yee G, et al. Differential adipogenic and inflammatory properties of small adipocytes in Zucker obese and lean rats. Diabet Vascul Dis Res 2010; 7: 311-8.
Ohsumi J, Sakakibara S, Yamaguchi J, et al. Troglitazone prevents the inhibitory effects of inflammatory cytokines on insulin-induced adipocyte differentiation in 3T3-L1 cells. Endocrinology 1994; 135: 2279-82.
Abbott WG, Foley JE. Comparison of body composition, adipocyte size and glucose and insulin concentration in Pima Indian and Caucasian children. Metabolism 1978; 36: 576-9.
Martin G, Schoonjans K, Staels B, Auwerx J. PPARgamma activators improve glucose homeostasis by stimulating fatty acid uptake in the adipocytes. Atherosclerosis 1998; 137: S75-80.
Stienstra R, Duval C, Keshtkar S, van der Laak J, Kersten S, Muller M. Peroxisome proliferator-activated receptor gamma activation promotes infiltration of alternatively activated macrophages into adipose tissue. J Biol Chem 2008; 283: 2620-7.
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