Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis

Joanna Szymańska-Kałuża; Barbara Cebula-Obrzut; Piotr Smolewski; Jerzy Stanczyk; Elżbieta Smolewska

doi:10.5114/ceji.2014.42128

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis

Joanna Szymańska-Kałuża

,

Barbara Cebula-Obrzut

,

Piotr Smolewski

,

Jerzy Stanczyk

,

Elżbieta Smolewska

(Centr Eur J Immunol 2014; 39 (1): 71-76)

DOI: https://doi.org/10.5114/ceji.2014.42128

Online publish date: 2014/04/22

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AMA

Szymańska-Kałuża J, Cebula-Obrzut B, Smolewski P, Stanczyk J, Smolewska E. Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central European Journal of Immunology. 2014;39(1):71-76. doi:10.5114/ceji.2014.42128.

APA

Szymańska-Kałuża, J., Cebula-Obrzut, B., Smolewski, P., Stanczyk, J.,  & Smolewska, E. (2014). Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central European Journal of Immunology, 39(1), 71-76. https://doi.org/10.5114/ceji.2014.42128

Chicago

Szymańska-Kałuża, Joanna, Barbara Cebula-Obrzut, Piotr Smolewski, Jerzy Stanczyk,  and Elżbieta Smolewska. 2014. "Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis". Central European Journal of Immunology 39 (1): 71-76. doi:10.5114/ceji.2014.42128.

Harvard

Szymańska-Kałuża, J., Cebula-Obrzut, B., Smolewski, P., Stanczyk, J.,  and Smolewska, E. (2014). Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central European Journal of Immunology, 39(1), pp.71-76. https://doi.org/10.5114/ceji.2014.42128

MLA

Szymańska-Kałuża, Joanna et al. "Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis." Central European Journal of Immunology, vol. 39, no. 1, 2014, pp. 71-76. doi:10.5114/ceji.2014.42128.

Vancouver

Szymańska-Kałuża J, Cebula-Obrzut B, Smolewski P, Stanczyk J, Smolewska E. Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central European Journal of Immunology. 2014;39(1):71-76. doi:10.5114/ceji.2014.42128.

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Objectives: The imbalance between Th17 and T regulatory cells (Tregs) may be a key event in development of autoimmunity. The problem is poorly explored in juvenile idiopathic arthritis (JIA) so far. In this study, peripheral blood (PB) and synovial fluid (SF) Tregs and Th17 cells from were assessed in untreated JIA children.

Material and methods: In 50 children with JIA the PB or SF percentages of Tregs and Th17 cells were assessed by flow cytometry, in comparison with PB Tregs and Th17 cells from 28 healthy controls. Additionally, in both groups the levels of proinfammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-17, IL-21, IL-23 and tumor necrosis factor α (TNF-α) were assessed using ELISA method.

Results: The proportion of JIA PB Th17 cells was significantly higher than in the controls (p = 0.01). Serum levels of IL-1β, IL-6, IL-17, IL-23 were also significantly higher in JIA (p = 0.011, p = 0.007, p = 0.008 and p = 0.023, respectively). The highest serum IL-6 levels were observed in oligoarthritis JIA (p = 0.031). Synovial fluid IL-21 concentration was distinctly higher in polyarticular JIA. Synovial fluid levels of TNF-α, IL-1β and IL-6 were significantly higher than in JIA PB (p = 0.038, p = 0.013 and p < 0.001, respectively). There was a significant correlation between IL-6 and PB Tregs (p = 0.02).

Conclusions: The results of this comprehensive analysis indicate a role of Th17 cell activation in the pathogenesis of JIA.

keywords:

Imbalance of Th17 and T-regulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis

Joanna Szymańska-Kałuża , Barbara Cebula-Obrzut , Piotr Smolewski , Jerzy Stanczyk , Elżbieta Smolewska

juvenile idiopathic arthritis, Th17 cells, T-regulatory cells, proinflammatory cytokines

Joanna Szymańska-Kałuża

,

Barbara Cebula-Obrzut

,

Piotr Smolewski

,

Jerzy Stanczyk

,

Elżbieta Smolewska