eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2019
vol. 44
 
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abstract:
Clinical immunology

Immunological characterization of the American cockroach allergen Per a 9 expressed in baculovirus-infected insect cells

Hai-Wei Yang
,
Wei Zhang
,
Wei-Wei Ni
,
Min Jin
,
Shao-Heng He
,
Ji-Fu Wei

(Centr Eur J Immunol 2019; 44 (3): 322-326)
Online publish date: 2019/09/30
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American cockroach (CR) allergy has been recognized as important IgE-mediated type I hypersensitivity. Per a 9 is an arginine kinase, reacting with IgE in sera of all CR allergic Thai patients. Per a 9 gene was cloned and expressed in eukaryotic systems (baculovirus-infected insect cells). The expressed Per a 9 was purified by Nickel column. The antigenicities were analyzed by ELISA, immunoblot analysis and basophile activation test. The results show that 13 out of 16 (81.3%) sera from American CR patients reacted to Per a 9, confirming that Per a 9 is a major allergen of CR. The IgE reactivity of Per a 9 in the sera from American CR patients was increased 8.3-fold in comparison with the sera from healthy controls. Per a 9 at 1.0 µg/ml induced an approximately up to 5.6-fold increase in CD63 and CCR3 double positive cells when incubating with passively sensitized basophils from by sera from American CR patients.
keywords:

American cockroach, allergen, Per a 9, insect cell, IgE reactivity

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