eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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vol. 42
Experimental research

Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Ibrahim Salameh Al-Majali
Sawsan Atallah Al-Oran
Mona Rushdie Hassuneh
Haitham Naief Al-Qaralleh
Walid Abu Rayyan
Osama Yosef Al-Thunibat
Eyad Mallah
Ahmed Abu-Rayyan
Shadi Salem

(Centr Eur Immunol 2017; 42 (3): 231-238)
Online publish date: 2017/10/30
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This study was conducted to assess the in vivo and ex vivo immunomodulatory effect of the ethanol leaves extract of Moringa peregrina in Balb/c mice. For this study, five groups of 5 Balb/c mice were given a single acute subtoxic oral dose of the ethanolic extract at 1.13, 11.30, 23.40 and 113.4 mg/kg and the immunomodulatory effect was assessed on the 6th day following the ingestion. In the (non-functional) assessment, the effect of the extract on the body weight, relative lymphoid organ weight, splenic cellularity and peripheral blood hematologic parameters were evaluated. While in the immunomodulation assessment (functional), we investigated the effect of the extract on the proliferative capacity of splenic lymphocytes and peripheral T and B lymphocytes using mitogen blastogenesis, mixed allogeneic MLR and IgM-Plaque forming cells assays. The ingestion of M. peregrina extract caused a significant increase in the body weight, weight and number of cells of spleen and lymph nodes of the treated mice. Furthermore, the count of RBCs, WBCs, platelets, hemoglobin concentration and PCV % were increased by the extract treatment in a dose-dependent manner. M. peregrina enhanced the proliferative responses of splenic lymphocytes for both T cell and B-cell mitogens. Likewise, the mixed lymphocyte reaction MLR assay has revealed a T-cell dependent proliferation enhancement in the extract treated mice. Moreover, the oral administration of M. peregrina leaves extracts significantly increased PFCs/106 splenocytes in a dose-dependent manner. In conclusion, subtoxic acute doses of M. peregrina extract demonstrated significant potential as an immunomodulatory agent even at the lowest dose of 1.13 mg/kg.

Moringa peregrina, immunomodulation, mouse spleen, T and B lymphocytes, body weight

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