eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2018
vol. 43
 
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abstract:
Experimental immunology

Influence of trans-resveratrol on macrophage and lymphocyte activity in rainbow trout (Oncorhynchus mykiss) – in vitro study

Elżbieta Terech-Majewska, Andrzej Krzysztof Siwicki, Sylwia Hermańska, Edyta Kaczorek-Łukowska, Joanna Pajdak-Czaus, Patrycja Schulz

(Centr Eur J Immunol 2018; 43 (3): 241-247)
Online publish date: 2018/10/30
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The objective of this study was to investigate the effect of trans-resveratrol, a potent antioxidant with anti-inflammatory and chemopreventive properties, naturally occurring in many fruits and plants on lymphocytes proliferation and also on macrophages metabolic and phagocytic activity. The aim of this study was to demonstrate the immunomodulatory effects of the compound on fish immunocompetent cells and determine the type of this interaction (immunosuppression or immunostimulation). Proliferative activity of lymphocytes was studied by MTT assay, and the respiratory burst was evaluated using the respiratory burst activity (RBA) test. Phagocytic killing was tested using the PKA test. The experiment have shown that trans-resveratrol suppressed blood B cells, while there was no significant influence on blood T lymphocytes. However, insignificant stimulatory effect occurred at the lowest concentration. In addition, the compound inhibited proliferation of T and B lymphocytes isolated from the organs. Importantly, trans-resveratrol caused stimulation of blood and organs macrophages phagocytic killing, and also increased the respiratory burst of macrophages isolated from organ. These results suggest a potential use of trans-resveratrol as an immunomodulator of innate immunity in fish. This is particularly important, as this kind of resistance plays leading role in protecting the body against infection. In comparison, adaptive immunity is slower and also much less precise.
keywords:

trans-resveratrol, in vitro studies, immunomodulators, cellular immunity

references:
Takaoka MJ (1940): The phenolic substances of white Hellebore (Veratrum grandiflorum Loes.fil). J Faculty Sci Hokkaido Imperial University 3: 1-16.
Langcake P, Pryce RJ (1976): The production of reveratrol by Vitis vinifera and Rother members of the Vitaceae as a response to infection or injury. Physiol Plant Pathol 9: 77-86.
King RE, Bomser JA, Min DB (2006): Bioactivity of Resveratrol. Compr Rev Food Sci Food Saf 5: 65-70.
Fremont L (2000): Biological effects of resveratrol. Life Sci 66: 663-673.
Tome-Carneiro J, Larrosa M, Gonzalez-Sarrias A, et al. (2013): Resveratrol and clinical trials: The crossroad in vitro studies to human evidence. Curr Pharm Design 19: 6064-6093.
Kimura Y, Okuda H, Arichi S (1985): Effects of stilbeneson arachidonate metabolism in leukocytes. Biochim Biophys Acta 834: 275-278.
Baur JA, Sinclair DA (2006): Therapeutic potential of resveratrol: the in vivo evidence, Nat Rev Drug Discov 5: 493-506.
Szkudelska K, Szkudelski T (2010): Resveratrol, obesity and diabetes. Eur J Pharmacol 635: 1-8.
Schirmer H, Pereira TC, Rico EP, et al. (2012): Modulatory effect of resveratrol on SIRT1, SIRT3, SIRT4, PGCL and NAMPT gene expression profiles in wild-type adult zebrafish liver. Mol Biol Rep 39: 3281-3289.
Aggarwal BB, Bhardwaj A, Aggarwal RS, et al. (2004): Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res 24: 2783-2840.
Baur JA, Pearson KJ, Price NL, et al. (2006): Resveratrol improves health and survival of mice on high-calorie diet. Nature 444: 337-342.
Howitz KT, Bitterman KJ, Cohen HY, et al. (2003): Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature 425: 191-196.
Ratan HL, Steward WP, Gescher AJ, Mellon JK (2002): Resveratrol  a prostate cancer chemopreventive agent? Urol Oncol 7: 223-227.
Timmers S, Auwerx J, Schrauwen P (2012): The journey of resveratrol from yeast to human. Aging 4: 146-158.
Valenzano DR, Terzibasi E, Genade T, et al. (2006): Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Curr Biol 16: 296-300.
Wood JG, Rogina B, Lavu S, et al. (2004): Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature 430: 686-689.
Sakai M, Kobayashi M, Kawauchi H (1996): In vitro activation of fish phagocytic cells by GH, prolactin and somatolactin. J Endocrinol 151: 113-118.
Siwicki AK, Anderson DP, Dunier M (1996): In vitro immunotoxicology-alternative models for environmental toxicology. In: Biologiczne Monitorowanie Skażenia Środowiska. IRS Olsztyn; 157-170.
Siwicki AK, Morand M, Klein P, et al. (1998): Modulation of nonspecific mechanisms and protection against diseases in Fish. Acta Vet Brno 67: 323-328.
Studnicka M, Siwicki AK, Morand M, et al. (2000): Modulation of nonspecific Demence mechanisms and specific immune responses after suppression induced by xenobiotics. J Appl Ichthyol 16: 1-7.
Kazuń K, Siwicki AK (2001): Propiscin – a safe new anaesthetic for fish. Arch Pol Fish 9: 183-190.
Chung S, Secombes S (1988): Analysis of events occurring within teleost macrophages during respiratory burst. Comp Biochem Physiol 89: 539-544.
Chettri JK, Holten-Andersen L, Buchmann K (2010): Factors influencing in vitro respiratory burst assays with head kidney leucocytes from rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 33: 593-602.
Rook GA, Steele J, Umar S, Dockrell HM (1985): A simple method for the solubilisation of reduced NBT and its use as a colorimetric assay for activation of human macrophages by gamma-interferon. J Immunol Methods 82: 161-167.
Małaczewska J, Siwicki AK, Wójcik R, et al. (2014): The in vitro effect of kynurenic acid on the rainbow trout (Oncorhynchus mykiss) leukocyte and splenocyte activity. Pol J Veter Scin 17: 453-458.
Mosmann T (1983): Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
Boscolo P, Del Signpre A, Sabbioni E, et al. (2003): Effects of resveratrol on lymphocyte proliferation and cytokine release. Ann Clin Lab Sci 33: 226-231.
Feng YH, Zhou WL, Wu QL, et al. (2002): Low dose of resveratrol enhanced immune response of mice. Acta Pharmacol Sin 23: 893-897.
Gao H, Deeb D, Media J, et al. (2003): Immunomodultaory activity of resveratrol: discrepant in vitro and in vivo immunological effects. Biochem Pharmacol 66: 2427-2435.
Sharma S, Chopra K, Kulkarni S (2007): Resveratrol and curcumin suppress immune responses through CD28/CTLA-4 and CD80 co-stimulatory pathway. Clon Exp Immunol 147: 155-163.
Poolman TM, Ng LL, Farmer PB, Manson MM (2005): Inhibition of the respiratory burst by resveratrol in human mocytes: Correlation with inhibition of PI3K signaling. Free Radic Biol Med 39: 118-132.
Deby-Dupont G, Mouithys-Mickalad A, Serteyn D, et al. (2005): Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells. Biochem Biophys Res Commun 333: 21-37.
Martinez J, Moreno JJ (200): Effect of resveratrol, a natural polyphenolic compound, on reactive oxygen species and prostaglandin production. Biochem Pharmacol 59: 865-870.
Leiro J, Alvarez E, García D, Orallo F (2002): Resveratrol modulates rat macrophage functions. Int Immunopharmacol 2: 767-774.
Iyori M, Kataoka H, Shamsul HM, et al. (2008): Resveratrol modulates phagocytosis of bacteria through an NF-B-
dependent gene program. Antimicrob Agents Chemother 52: 121-127.
Ragione FD, Cucciolla V, Borriello A, et al. (1998): Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem Biophys Res Commun 250: 53-58.
Fontecave M, Lepoivre M, Elleingand E, et al. (1997): Resveratrol, a remarkable inhibitor of ribonucleitide reductase. FEBS Lett 421: 277-279.
Sun NJ, Woo SH, Cassady JM, Snapka RM (1998): DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia. J Nat Prod 61: 362-366.
Gao X, Xu YX, Janakiraman N, et al. (2001): Immunomodulatory activity of resveratrol: suppression of lymphocyte proliferation, development of cell-mediated cytotoxicity, and cytokine production. Biochem Pharmacol 62: 1299-1308.
Zunino SJ, Storms DH (2009): Resveratrol alters proliferative responses and apoptosis in human activated B lymphocytes in vitro. J Nutr 139: 1603-1608.
Sharma S, Anjaneyulu M, Kulkarni SK, Chopra K (2006): Resveratrol, a polyphenolic phytoalexin, attenuates diabetic nephropathy in rats. Pharmacology 76: 69-75.
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