eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

3/2018
vol. 43
 
Share:
Share:
more
 
 
abstract:
Experimental immunology

Influence of trans-resveratrol on macrophage and lymphocyte activity in rainbow trout (Oncorhynchus mykiss) – in vitro study

Elżbieta Terech-Majewska
,
Andrzej Krzysztof Siwicki
,
Sylwia Hermańska
,
Edyta Kaczorek-Łukowska
,
Joanna Pajdak-Czaus
,
Patrycja Schulz

(Centr Eur J Immunol 2018; 43 (3): 241-247)
Online publish date: 2018/10/30
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
The objective of this study was to investigate the effect of trans-resveratrol, a potent antioxidant with anti-inflammatory and chemopreventive properties, naturally occurring in many fruits and plants on lymphocytes proliferation and also on macrophages metabolic and phagocytic activity. The aim of this study was to demonstrate the immunomodulatory effects of the compound on fish immunocompetent cells and determine the type of this interaction (immunosuppression or immunostimulation). Proliferative activity of lymphocytes was studied by MTT assay, and the respiratory burst was evaluated using the respiratory burst activity (RBA) test. Phagocytic killing was tested using the PKA test. The experiment have shown that trans-resveratrol suppressed blood B cells, while there was no significant influence on blood T lymphocytes. However, insignificant stimulatory effect occurred at the lowest concentration. In addition, the compound inhibited proliferation of T and B lymphocytes isolated from the organs. Importantly, trans-resveratrol caused stimulation of blood and organs macrophages phagocytic killing, and also increased the respiratory burst of macrophages isolated from organ. These results suggest a potential use of trans-resveratrol as an immunomodulator of innate immunity in fish. This is particularly important, as this kind of resistance plays leading role in protecting the body against infection. In comparison, adaptive immunity is slower and also much less precise.
keywords:

trans-resveratrol, in vitro studies, immunomodulators, cellular immunity

FEATURED PRODUCTS
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe