eISSN: 2084-9834
ISSN: 0034-6233
Reumatologia/Rheumatology
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5/2020
vol. 58
 
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abstract:
Original paper

Investigation of anti-inflammatory effects of bee venom in experimentally induced adjuvant arthritis

İbrahim Tekeoğlu
1
,
Mehmet Akdoğan
2
,
İlhami Çelik
3

1.
Department of Rheumatology/Rehabilitation, Research and Teaching Hospital, Sakarya University, Turkey
2.
Department of Medical Biochemistry, Medical Faculty, Sakarya University, Turkey
3.
Department of Histology and Embryology, Veterinary Faculty, Selçuk University, Turkey
Reumatologia 2020; 58, 5: 265–271
Online publish date: 2020/10/15
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Introduction
Rheumatoid arthritis is a multisystemic inflammatory disease characterized by destruction of the joints. An effective treatment method of the disease has not been developed yet. The aim of the present study is to evaluate the effects of bee (Apis mellifera anatoliaca) venom (BV) on serum inflammatory parameters, serum antioxidant load and clinical parameters of experimentally induced adjuvant arthritis in rats.

Material and methods
A total of 35 Wistar albino male rats were used. The animals were divided into 5 groups. First group animals served as negative controls. The second, third, fourth and fifth groups were used for experimental arthritis induction. Following clinical development of arthritis, the first group was subcutaneously administered 0.2 ml of physiological saline, and the second, third and fourth groups were treated subcutaneously with 2 µg/kg, 4 µg/kg and 20 µg/kg once a week three times. Physiological saline injected fifth group animals were used as a sham-treatment group. Clinical observations and evaluation of arthritis were made at the 15th day, and at the end of the experiment. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, paraoxonase, serum aryl esterase, high-sensitivity C reactive protein, interleukin 1 (IL-1), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-) were determined in cardiac blood samples taken at the end of the 29th day.

Results
From the data, total oxidant level (TOL) and oxidative stress index (OSI) were calculated. Significant improvements were observed in the clinical signs of arthritis and inflammatory markers such as in IL-1, TNF-, IL-6 and TOL and OSI in the 20.0 µg/kg BV-administered group. Bee venom administration did not cause any significant increase in ALT and AST values or signs of liver toxicity.

Conclusions
Bee venom treatment was effective in alleviation of symptoms of the experimental rat adjuvant arthritis by means of clinical observation and serum inflammatory markers.

keywords:

adjuvant arthritis, bee venom, total oxidant status, oxidative stress index






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