Hepatocyte growth factor (HGF) can be produced by various cells and elicits multiple biological responses such as motility, proliferation, morphogenesis and survival in a cell type-dependent fashion. Apart from its physiological importance, HGF may contribute to many organ diseases [1-3]. We have already shown that conversely to routinely measured biomarkers of myocardial injury, human HGF (hHGF) increases to high levels in the very early phase of acute coronary syndrome (ACS) and then decreases rapidly during the first 24 h of ACS [4]. As described in our previous article, we measured hHGF concentration only twice, namely at admission and 24 h later [4]. We decided to establish the dynamicity of hHGF level changes on the first day of myocardial injury and to determine the usefulness of its measurement in the case of untimely complications.
In 4 patients with ST-segment elevation myocardial infarction (STEMI) and in 2 patients with non ST-segment elevation myocardial infarction (NSTEMI), who were eligible for coronary angiography and, possibly, primary percutaneous coronary intervention (PPCI), hHGF measurements were repeated 10 times during the first day of ACS and once at discharge from hospital (Figure 1). Each subject expressed their informed consent to participate in the study. Patients’ informed consent and the protocol of the study were approved by the Institutional Local Ethics Committee. Patients’ detailed particulars are presented in the Table I.
Human HGF was determined in plasma using the Quantikine Elisa kit from R&D Systems, Minneapolis, MN, USA. The mean value of hHGF evaluated in EDTA plasma using the Quantikine Elisa kit and presented in the manufacturer’s brochure as a reference value was 787 pg/ml (range: 469-1113 pg/ml).
In the first measurement, as illustrated in the Figure 1, a marked increase in hHGF levels was observed in STEMI patients. Very high values (maximally about 20 000 pg/ml) were reduced to almost normal values within 5 h from the first measurement. In NSTEMI patients, even in the first assessment we did not observe very high levels of hHGF, and during the first 24 h of ACS, hHGF levels were stable. Contrary to patients with an uncomplicated acute course of STEMI, 2 patients with serious cardiovascular events in the acute stage of myocardial infarction, namely cardiac arrest and early reocclusion of the coronary artery, revealed a second increase of hHGF levels during the...
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