eISSN: 1896-9151
ISSN: 1734-1922
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abstract:
Basic research

MiR-345-5p inhibits tumorigenesis of papillary thyroid carcinoma by targeting SETD7

Ming Zhao
,
Kejing Wang
,
Jinbiao Shang
,
Zhong Liang
,
Weihui Zheng
,
Jialei Gu

Online publish date: 2019/03/25
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Introduction
This study aimed to explore the effects of miR-345-5p on papillary thyroid carcinoma (PTC) and uncover the possible mechanisms.

Material and methods
MiR-345-5p and SETD7 mRNA levels were analyzed by quantitative real-time PCR and SETD7 protein level was measured by Western blot. The viability, colony formation ability and apoptosis of PTC cells were measured with CCK-8, soft agar colony formation and flow cytometry assay, respectively. Luciferase reporter assay was used to identify miR-345-5p’s target.

Results
Compared to neighboring normal tissues, there was lower miR-345-5p expression and higher SETD7 expression in PTC tissues. Moreover, Spearman’s correlation analysis indicated that there was a negative correlation between miR-345-5p and SETD7 expression in PTC tissues. MiR-345-5p mimics inhibited the viability and colony formation of TPC1 and B-CPAP cells and promoted apoptosis, whereas anti-miR-345-5p promoted PTC cell proliferation and inhibited apoptosis. SETD7 was confirmed to be a direct target of miR-345-5p through target scan analysis and luciferase reporter assay. Additionally, overexpression of SETD7 promoted the viability and colony formation of TPC1 and B-CPAP cells and inhibited apoptosis, whereas downregulation of SETD7 by shRNAs had opposite effects on PTC cells. Furthermore, overexpression of SETD7 attenuated the miR-345-5p induced anti-tumor effects on PTC cells.

Conclusions
MiR-345-5p exhibited suppressive effects on PTC via targeting SETD7.

keywords:

miR-345-5p, papillary thyroid carcinoma, proliferation, apoptosis, SETD7

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