eISSN: 1896-9151
ISSN: 1734-1922
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abstract:
Basic research

MicroRNA-101 inhibits growth and metastasis of human ovarian cancer cells by targeting PI3K/AKT

Min Wei
,
Hongjuan Jin
,
ShuLi Yang
,
Zhuo Li
,
Xinlei Wang
,
LiXiang Li
,
Yan Jia
,
ManHua Cui

Online publish date: 2019/05/28
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Introduction
Ovarian cancer is the most frequent cause of gynecological cancer related mortality in woman. This study was designed to investigate the role and therapeutic potential of miRNA-101 in ovarian cancer.

Material and methods
Expression analysis was carried out by real-time quantitative polymerase chain reaction. Transfections were performed with the help of Lipofectamine 2000 reagent. AO/EB and annexin V/PI staining was used to detect apoptosis and flow cytometry was used for cell cycle analysis. Western blotting was employed for cell cycle analysis.

Results
It was found that miRNA-101 was significantly down-regulated in ovarian cancer cells. The over-expression of miRNA-101 causes a significant decrease in the viability of ovarian cancer cells via the initiation of apoptosis and sub-G1 arrest of OVACAR-3 cells. It was indicated that PTEN was the potential target of miRNA-101 in OVACAR-3 cells. There was 4.5-fold up-regulation of PTEN expression in ovarian cancer cell lines and the over-expression of miRNA-101 in OVACAR-3 cells resulted in the down-regulation of PTEN expression. The inhibition of PTEN in the OVACAR-3 cells arrested the proliferation of these cells. The over-expression of miRNA-101 causes significant down-regulation in PI3K and AKT expression of OVACAR-3 cells.

Conclusions
It can be concluded that miRNA-101 acts as a tumor suppressor which may be beneficial in the treatment of ovarian cancer.

keywords:

ovarian cancer, apoptosis, microRNA, proliferation

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