eISSN: 1896-9151
ISSN: 1734-1922
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abstract:
Basic research

MicroRNA-214-5p protects against myocardial ischemia reperfusion injury through targeting the FAS ligand

Yuan Lu
,
Jue Xi
,
Yao Zhang
,
Chenzong Li
,
Wensu Chen
,
Xiaoqin Hu
,
Min Zhang
,
Fengyun Zhang
,
Hui Wei
,
Zhi Li
,
Zhirong Wang

Online publish date: 2019/05/28
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Introduction
MicroRNAs (miRNAs) are considered as crucial modulators in myocardial ischemia and reperfusion (I/R) injury. The present study aimed to investigate the expression and biological functions of miR-214-5p via targeting Fas ligand (FASLG) in I/R injury.

Material and methods
Lactate dehydrogenase, casein kinase, malondialdehyde assay, reactive oxygen species (ROS) detection and cell apoptosis analysis measured cell damage and cell apoptosis in H9c2 cells under hypoxia/reperfusion (H/R) treatment. Bioinformatics and dual luciferase reporter assays demonstrated the molecular mechanism of miR-214-5p in cardiac cells. 2,3,5-Triphenyltetrazolium chloride (TTC) staining, hematoxylin-eosin (HE) staining and adenovirus injection were performed in I/R treated mice.

Results
The expression of miR-214-5p was decreased in H/R injured H9c2 cells compared with control cells (p < 0.001). Overexpression of miR-214-5p reduced cell damage and apoptosis in H9c2 cells under H/R treatment (p < 0.001). Further study revealed that FASLG was a target of miR-214-5p. Enhanced expression of FASLG attenuated the protective function of miR-214-5p in H9c2 cells subjected to H/R injury (P < 0.001). Moreover, the elevated expression of miR-214-5p by adenovirus injection protected cardiac cells from I/R injury in mice (n = 6/per group).

Conclusions
We found that miR-214-5p exerted a protective role in I/R injured cardiac cells by direct targeting FASLG in vitro and in vivo.

keywords:

ischemic heart diseases, miR-214-5p, FASLG, cell apoptosis

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