eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
Current issue Archive Manuscripts accepted About the journal Editorial board Journal's reviewers Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
3/2021
vol. 38
 
Share:
Share:
more
 
 
abstract:
Original paper

Modulation of dermal equivalent of hypothalamuspituitary- adrenal axis in mastocytosis

Jakub Antoniewicz
1
,
Bogusław Nedoszytko
2
,
Magdalena Lange
2
,
Justyna Wierzbicka
1
,
Magdalena Górska-Ponikowska
3
,
Marek Niedoszytko
4
,
Monika Zabłotna
2
,
Roman J. Nowicki
2
,
Michał A. Żmijewski
1

1.
Department of Histology, Medical University of Gdansk, Gdansk, Poland
2.
Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland
3.
Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland
4.
Department of Pneumatology and Allergology, Medical University of Gdansk, Gdansk, Poland
Adv Dermatol Allergol 2021; XXXVIII (3): 461–472
Online publish date: 2021/07/26
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Introduction
Mastocytosis is a rare disease characterized by abnormal growth and accumulation of tissue mast cells (MC) in one or more organ systems and is classified as being either cutaneous mastocytosis (CM) or systemic mastocytosis (SM). In the pioneer studies of Slominski’s group, a fully functional hypothalamic-pituitary-adrenal axis equivalent has been discovered in various tissues, including skin.

Aim
In the present study we investigated potential involvement of hypothalamus-pituitary-adrenal (HPA) cutaneous equivalent in ongoing mastocytosis.

Material and methods
The expression of HPA elements: CRH, UCN1, UCN2, UCN3, CRHR1, POMC, MC1R, MC2R and NR3C1 was assessed for their mRNA level in skin biopsies of adult patients with mastocytosis and healthy donors (n = 16 and 19, respectively), while CRH, UCN1, CRHR1, ACTH and MC1R were selected for immunostaining assay (n = 13 and 7, respectively). The expression of CRH receptor 1 (CRHR1) isomers was investigated by RT-PCR. The ELISA was used for detection of cortisol, CRH, UCN and ACTH in the serum.

Results
The decrease in the expression of HPA element of skin equivalent was observed on both mRNA and protein levels, however quantification of immunohistochemical data was impeded due to melanin in epidermis. Furthermore, we observed infiltration of dermis with HPA elements overexpressing mononuclear cells, which is in the agreement with an in vitro study showing a high expression of HPA elements by mast cells.

Conclusions
Taken together, it was confirmed that the expression elements of HPA was modulated in mastocytosis, thus the potential involvement of general and local stress responses in its pathogenesis should be postulated and further investigated.

keywords:

skin hypothalamic-pituitary-adrenal axis, mastocytosis, mast cells, corticotrophin-releasing hormone, adrenocorticotropic hormone, corticotrophin-releasing hormone receptor

Quick links
© 2021 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe