Abstract
Germinal polymorphism of methionine synthetase gene in the aspect of red meat consumption and colorectal cancer risk – preliminary report
- Institute of Medical Sciences, Jan Kochanowski University, Kielce, Poland
- Institute of Health Sciences, Jan Kochanowski University, Kielce, Poland
- Department of Pathomorphology, State Medical Institute of the Ministry of Interior and Administration in Warsaw, Warsaw, Poland
Introduction:
Methionine synthase is a vitamin B12-dependent enzyme involved in cyclic re-synthesis methionine from homocysteine. To date, the research with MTR A2756G (rs1805087) variant and risk of cancers brought the opposite results. It was also shown that the relationship with environmental factors such as diet and genetic predisposition play an important role in cancer development, including colorectal cancer (CRC).
Aim of the research:
To analyse the association of MTR variants with CRC in respect of dietary patterns and health conditions.
Material and methods:
The prevalence of MTR variants was determined in 191 patients with colorectal cancer and 93 healthy volunteers. The DNA was isolated from blood (MagCore II, RBC Bioscience). Genotypes and allele frequencies were determined in patients and controls using TaqMan SNP genotyping assays (ThermoFischer Scientifics).
Results:
There were no cases of MTR rs121913582, rs121913580, or rs121913578 mutations in CRC or controls. There is no correlation between CRC occurrence and MTR c.2756A>G genotype presence. We confirm that red meat consumption increases the risk of CRC. There was no statistically significant correlation between environmental factors and genetic variants.
Conclusions:
Red meat consumption greater than 50 g/day increase the risk of colorectal cancer by approximately 20%. There is no correlation between MTR variant and CRC risk as well as CRC localisation.
>Keywords
colorectal cancer, red meat consumption, methionine synthetase
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