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Lipid disorders
Research letter

Statins and C-reactive protein: in silico evidence on direct interaction

Neda Shakour
1
,
Massimiliano Ruscica
2
,
Farzin Hadizadeh
3
,
Cesare Cirtori
4
,
Maciej Banach
5, 6
,
Tannaz Jamialahmadi
7, 8
,
Amirhossein Sahebkar
3, 9, 10

1.
Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2.
Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy
3.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4.
Centro Dislipidemie, A.S.S.T. Grande Ospedale Metropolitano Niguarda, Milan, Italy
5.
Department of Hypertension, WAM University Hospital, Medical University of Lodz, Lodz, Poland
6.
Polish Mother’s Memorial Hospital Research Institute (PMMHRI), Lodz, Poland
7.
Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran
8.
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
9.
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
10.
Halal Research Center of IRI, FDA, Tehran, Iran
Arch Med Sci 2020; 16 (6): 1432–1439
Online publish date: 2020/11/02
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C-reactive protein (CRP) is a liver protein associated with inflammation. Being readily assayable in the circulation it has an established clinical role in the evaluation of the inflammatory status in generalized inflammatory diseases. Among these, foremost is rheumatoid arthritis, followed by chronic diseases such as spondylitis, lupus and others [1, 2]. CRP levels rise after any microbial caused inflammation, from tonsils to subcutaneous tissues, as well as after traumas [3].

The major novelty in the field of inflammatory disorders has been the recognition that generalized changes associated with CRP levels 40–200 mg/l are just one face of the pathophysiological role of CRP [4]. Indeed, detection of CRP rises only measurable by high-sensitivity testing (hs-CRP) provided unexpected information on the role of hs-CRP as a marker of diseases not at the classical inflammation sites, but rather at the cardiovascular level [5].

Hs-CRP > 2 mg/l is associated with major cardiovascular risk markers, such as elevated lipids, in particular low-density lipoprotein (LDL)-cholesterol, as well as in general with the progression of cardiovascular lesions [6]. In this particular case, hs-CRP > 2 mg/l becomes a major marker for early detection of arterial lesions and, more importantly, for the monitoring of agents reducing cardiovascular risk, in particular lipid-lowering medications. Clinical studies on the major HMG-CoA reductase inhibitors, i.e. statins, have shown that patients with coronary disease not associated with marked hypercholesterolemia benefit from the reduction of this inflammatory marker induced by statins [7].

Recently questions have been raised on the actual biomarker role of CRP after a study on genetic loci associated with hs-CRP levels failed to detect a clear association between these and the occurrence of CV events, in contrast to neighboring loci such as IL-6R or the APOCI-CII cluster [8]. A later Mendelian randomization study [9] from a coronary heart disease genetic collaboration, investigating individuals from 47 epidemiological studies in 15 countries, detected four genes tagging single nucleotide polymorphisms in the CRP gene. Variants of these were associated with an up to 30% per allele difference in hs-CRP concentrations but were unrelated to other coronary risk factors. None of these alleles was additive to the classical risk scores for coronary heart disease. In spite of the altogether not informative genetic characterization, it is unquestionable that the reduction of hs-CRP is associated with a definite CV benefit. Indeed, by using canakinumab, a selective antagonist of interleukin-1b in the CANTOS Study on post-myocardial infarction patients, a clear benefit in cardiovascular risk was associated with a highly significant (-35–40%) reduction of hs-CRP [10].

In the still ongoing debate on the clinical significance of CRP reduction induced by lipid-lowering or other treatments, the newly developed PCSK9 antagonists offer a good case in point. The major end point trials do not indicate anti-inflammatory activity of either bococizumab [11] or evolocumab [12], which may be responsible for the observed coronary prevention. The impressive LDL-cholesterol lowering induced by these agents was not associated with changes in hs-CRP levels [13]. These findings confirm the results of a careful meta-analysis from many trials with PCSK9i, showing a lack of effect on CRP levels [14]. An effect of these drugs on tissue cholesterol reduction, leading to a lack of inflammasome activation consequent to cholesterol crystal deposition [15], would thus not be supported. This major difference between statins, leading to reduced tissue cholesterol and lower inflammasome activation [16], and PCSK9 antagonists, has been underlined in recent reports attempting to explain the mortality difference in statin vs. PCSK9 trials [17] and the clearly reduced mortality in the CANTOS trial [8].

Since at present only indirect antagonist action of statins on CRP production has been hypothesized [18], i.e. consequent to inhibited protein geranylgeranyltransferase [19], a mechanistically more effective antagonism of the CRP activity as exerted by statins should be evaluated. This will be of major importance for the definition of an active site of CRP binding, allowing one to hypothesize the molecular structure of agents potentially able to inhibit the activity of CRP. In order to achieve this goal, active ligands, i.e. statins, were studied and compared to phosphorylcholine, the standard ligand of CRP [20]. Appropriate docking experiments were designed showing that all statins and phosphorylcholine could directly interact with CRP in the active site, providing reliable information on the most appropriate design of drugs potentially antagonizing CRP. In addition, the present study could potentially clarify whether the antagonist action on the CRP docking site may go in parallel with the well-assessed dose-related plasma LDL-C reduction or may be independent activity.

Methods

The orientation of the respective ligands and phosphorylcholine in the CRP active site was examined by a Molecular Operating Environment (MOE, Chemical Computing Group Inc. Montreal, http://www.chemcomp/com) docking experiment (Table I). Phosphorylcholine bound to CRP crystal structure was downloaded from the RCSB Protein Data Bank (PDB entry: 1B09). All the computational procedures were carried out with MOE. The molecular structures of the fluvastatin, pravastatin, pitavastatin, rosuvastatin, atorvastatin, simvastatin and lovastatin and phosphorylcholine (as the standard ligand) were prepared by MOE Builder and minimized energy was calculated using Hamiltonian-Force Field-MMFF94x by MOE. The docking procedure was performed with the default settings of the MOE-DOCK. The final docking scores were evaluated using the Generalized-Born Volume Integral/Weighted Surface area (GBVI/WSA) dG scoring function with the GBVI [21]. The GBVI/WSA dG is a forcefield-based scoring function, which estimates the free energy of binding of the ligand from a given pose. The dissociation constant (Ki) was computed through the binding free energy estimated with the GBVI/WSA dG scoring function according to the following equation: ΔG = RTln(Ki ), where R represents the gas constant and T the temperature in kelvin. The Ki was computed from the binding free energy values at a fixed temperature (300 K).

Table I

Right column – 3D-docking of phosphorylcholine and respective ligands with the CRP molecular target, Ca2+ (brown) is seen at the center. Left column – Docking of CRP with a-h compounds in 2D diagram. A – rosuvastatin, B – fluvastatin, C – pitavastatin, D – atorvastatin, E – phosphorylcholine, F – pravastatin, G – simvastatin, H – lovastatin

LigandsDocking 3D representationDocking 2D representation
(A)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i001_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i002_min.jpg
(B)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i003_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i004_min.jpg
(C)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i005_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i006_min.jpg
(D)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i007_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i008_min.jpg
(E)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i009_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i010_min.jpg
(F)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i011_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i012_min.jpg
(G)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i013_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i014_min.jpg
(H)/f/fulltexts/AOMS/42213/AMS-16-6-42213-i015_min.jpg/f/fulltexts/AOMS/42213/AMS-16-6-42213-i016_min.jpg
/f/fulltexts/AOMS/42213/AMS-16-6-42213-i017_min.jpg

Results

We studied the affinity of seven statins (rosuvastatin, fluvastatin, pitavastatin, atorvastatin, pravastatin, simvastatin and lovastatin) as well as the standard ligand phosphorylcholine to CRP. Docking experiments showed that all statins could directly interact with CRP (Table I). Among statins, rosuvastatin had the strongest interaction with CRP (pKi = 16.14) through one H bonding interaction of its carbonyl group to ASN_61 residues of CRP, followed by fluvastatin (pKi = 15.58; two H bonding interactions of its hydroxyl group to ASP_60 and ASN_61 residues), pitavastatin (pKi = 15.26; two H bonding interactions of its hydroxyl group to GLN_150 and ASN_61 residues), atorvastatin (pKi = 14.68; one H bonding interaction of its carbonyl group to GLN_150 residue), pravastatin (pKi = 13.95; three H bonding interactions of its hydroxyl group to GLN_150, ASN_61 and GLU_81 residues), simvastatin (pKi = 7.98; two H bonding interactions of its hydroxyl group to ASN_61 and ASP_140 residues), and lovastatin (pKi = 7.10; one H bonding interaction of its hydroxyl group to GLN_150 residue) (Table II). The standard ligand phosphorylcholine had interaction with CRP with a pKi value of 14.55 through two H bonding interactions of its hydroxyl group to GLN_150 and ASN_61 residues (Table I). Docking results indicated that each of the ligands [A-H] had an H-bonding interaction between the oxygen atom and ASN_61 residue except atorvastatin and lovastatin. Likewise, all ligands [A-H] had an H-bonding interaction between the oxygen atom and GLN-150 residue except simvastatin, fluvastatin and rosuvastatin (Table I). According to the above-mentioned results, rosuvastatin, fluvastatin, pitavastatin and atorvastatin with pki values of 16.14, 15.58, 15.26 and 14.68 were found to have stronger binding to CRP compared with the standard ligand phosphocholine (pKi = 14.55).

Table II

Data for the docking interactions of phosphorylcholine and statin molecules at the active sites of C-reactive protein (CRP) as the molecular target

No. of target protein (PDB-ID)LigandDocking scorepKiH–bonding interactions with amino acid residues
1B09Rosuvastatin–22.077716.14ASN_61
Fluvastatin–21.311615.58ASP_60
ASN_61
Pitavastatin–20.873915.26ASN_61
GLN_150
Atorvastatin–20.089614.68GLN_150
XRay Phosphorylcholine–19.910214.55ASN_61
GLN_150
Pravastatin–19.090013.95ASN_61
GLN_150
GLU_81
Simvastatin–10.91977.98ASN_61
ASP_140
Lovastatin–9.72237.10GLN_150

Discussion

The effect of lipid-lowering drugs on inflammation is receiving growing interest, particularly after the observation that PCSK9 antagonists, apparently devoid of significant antiinflammatory activity, at least as witnessed by a lack of reduction of hs-CRP [14], also affect to a lesser extent fatal CV events vs. statins [13, 17]. The present study, based on an in silico approach by molecular docking, on the interaction between CRP and different statins on the putative binding site of CRP is thus of potential high clinical interest and may lead to the development of newer agents, statins or others, with more powerful activity in binding inhibition.

A major observation stemming from the present study is that the inhibitory action of major statins on the CRP binding site appears to be to a large extent related to the lipid-lowering power of these molecules. As reported in a very recent review on the correlation between cholesterol lowering and inflammatory changes [22], CRP reductions occurring after statin treatment in trials of different length ranged between –9.8 and 89-3%. Interestingly, the largest reductions occurred in a study with either atorvastatin or pravastatin being given to individuals with extreme CRP elevations [23]. It should be noted that in this last quoted study published in 2005, CRP levels were apparently not evaluated by a high sensitivity assay; CRP reductions were, respectively –89.3 for atorvastatin and –82.4 for pravastatin. It is important to note that when evaluating studies on high-intensity statins, these gave the highest CRP reductions: in a direct comparative study on an IVUS evaluation of coronary plaque progression, atorvastatin treatment reduced CRP by 35.6% vs. 5.2% for pravastatin (p < 0.01) [24]. In the 2-year JUPITER study on rosuvastatin, the statin with the highest power, CRP, evaluated by the hs technique, was lowered by 57.1% [25]. Finally, a lower percent reduction of CRP (–27%) was found in the very large Heart Protection Study investigating the preventive activity of simvastatin [26]. In this study, differently from others, the reduction in vascular death appeared to be independent of baseline CRP levels. Another study evaluating effects of atorvastatin vs. simvastatin on atherosclerosis progression showed that hs-CRP after 1 year of treatment was decreased by 44.9% in the atorvastatin 80 mg group vs. 14.0% in the simvastatin 40 mg group. The same trend was maintained until the end of the study: –40.1% in the atorvastatin 80 mg group vs. 19.7% in the simvastatin 40 mg group [27].

Docking studies may be thus of crucial value since up to now no real correlation studies have monitored hs-CRP reduction vis-à-vis the chemical structure and inhibition of docking activity of statins for the CRP putative binding site. As shown by Kinlay [28], in a meta-analysis including 23 studies with statins, 89–98% of the CRP reduction (mean –28%) was related to the degree of LDL-C lowering; however, the event reduction was more significantly related to the reduction of LDL-C.

Finally, while there is no doubt that statins can improve systemic and vascular inflammation, also based on genome-wide association studies, controversial findings indicate that CRP may or may not play a significant role in the pathogenesis of atherosclerosis and particularly of acute CAD manifestations. The genome-wide association study [8] showed a lack of concordance between the effects on coronary risk of CRP genotypes and CRP levels, arguing against a causal association of CRP with coronary disease. Very recently Diederichsen et al. [29] in a vascular prediction study in 1,179 middle-aged subjects, concluded that, when adjusted for traditional risk factors and coronary calcium score, hs-CRP did not appear to add significantly to risk prediction.

In addition to CRP reduction or inhibited CRP binding, other anti-atherogenic mechanisms of statins should not be forgotten. Statins possess numerous pleiotropic actions [3042] and the relative role of each one of these in coronary prevention is difficult to establish. Hs-CRP, because of the relative straightforward determination and clear association with CAD risk, is a well-established CV risk marker, and inhibitory mechanisms may certainly prove beneficial.

The present study is limited by its in silico nature which necessitates further supportive evidence from experimental studies. In addition, future studies are warranted to test whether any direct interaction between statins and CRP structures could be considered at the protein binding sites other than the phosphorylcholine binding site.

In conclusion, the present study evaluated, by a docking technology, the inhibition of the binding of CRP to its putative active side by different statins. This led to a classification of statins by power of inhibitory activity, apparently in the same range as the CRP reduction associated with the lowering of CAD events. A limitation of the present study is obviously that the inhibitory action of statins on CRP binding to its site is linked to the amount of statins reaching this site in vivo, information not available by means of the present technology and, in addition, to the individual metabolism of statins allowing them to reach this site. Despite this limitation the study has the innovative potential of providing a tool for the study of different molecules, assessing their inhibitory effect on CRP binding, and thus of clear value in cardiovascular event reduction.

Acknowledgments

Neda Shakour and Massimiliano Ruscica contributed equally to this study.

Conflict of interest

The authors declare no conflict of interest.

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1. Otterness IG. The value of C-reactive protein measurement in rheumatoid arthritis. Semin Arthritis Rheum 1994; 24: 91-104.
2. Stumpf C, Sheriff A, Zimmermann S, et al. C-reactive protein levels predict systolic heart failure and outcome in patients with first ST-elevation myocardial infarction treated with coronary angioplasty. Arch Med Sci 2017; 13: 1086-93.
3. Ticinesi A, Lauretani F, Nouvenne A, et al. C-reactive protein (CRP) measurement in geriatric patients hospitalized for acute infection. Eur J Intern Med 2017; 37: 7-12.
4. Shadick NA, Cook NR, Karlson EW, et al. C-reactive protein in the prediction of rheumatoid arthritis in women. Arch Intern Med 2006; 166: 2490-4.
5. Tsimikas S, Willerson JT, Ridker PM. C-reactive protein and other emerging blood biomarkers to optimize risk stratification of vulnerable patients. J Am Coll Cardiol 2006; 47: C19-31.
6. Koenig W. High-sensitivity C-reactive protein and atherosclerotic disease: from improved risk prediction to risk-guided therapy. Int J Cardiol 2013; 168: 5126-34.
7. Everett BM, Glynn RJ, MacFadyen JG, Ridker PM. Rosuvastatin in the prevention of stroke among men and women with elevated levels of C-reactive protein: justification for the Use of Statins in Prevention: an Intervention Trial Evaluating Rosuvastatin (JUPITER). Circulation 2010; 121: 143-50.
8. Elliott P, Chambers JC, Zhang W, et al. Genetic loci associated with C-reactive protein levels and risk of coronary heart disease. JAMA 2009; 302: 37-48.
9. C Reactive Protein Coronary Heart Disease Genetics Collaboration (CCGC); Wensley F, Gao P, Burgess S, et al. Association between C reactive protein and coronary heart disease: mendelian randomisation analysis based on individual participant data. BMJ 2011; 342: d548.
10. Ridker PM, Everett BM, Thuren T, et al. Antiinflammatory therapy with canakinumab for atherosclerotic disease. N Engl J Med 2017; 377: 1119-31.
11. Ridker PM, Revkin J, Amarenco P, et al. Cardiovascular efficacy and safety of bococizumab in high-risk patients. N Engl J Med 2017; 376: 1527-39.
12. Raal FJ, Stein EA, Dufour R, et al. PCSK9 inhibition with evolocumab (AMG 145) in heterozygous familial hypercholesterolaemia (RUTHERFORD-2): a randomised, double-blind, placebo-controlled trial. Lancet 2015; 385: 331-40.
13. Ridker PM. Mortality differences associated with treatment responses in CANTOS and FOURIER: insights and Implications. Circulation 2018; 137: 1763-6.
14. Sahebkar A, di Giosia P, Stamerra A, et al. Effect of monoclonal antibodies to PCSK9 on high-sensitivity C-reactive protein levels: a meta-analysis of 16 randomized controlled treatment arms. Br J Clin Pharmacol 2016; 81: 1175-90.
15. Duewell P, Kono H, Rayner KJ, et al. NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals. Nature 2010; 464: 1357-61.
16. Wang S, Xie X, Lei T, et al. Statins attenuate activation of the NLRP3 inflammasome by oxidized-LDL or TNF-αlpha in vascular endothelial cells through a PXR-dependent mechanism. Mol Pharmacol 2017; 92: 256-64.
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