eISSN: 1896-9151
ISSN: 1734-1922
Archives of Medical Science
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5/2020
vol. 16
 
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Urology
abstract:
Basic research

Up-regulation of DRAM2 promotes tolerance of bladder transitional cell carcinoma to gemcitabine

Baihetiya Azhati
1
,
Naibijiang Maolakuerban
1
,
Tao Ma
1
,
Xiaodong Li
1
,
Mulati Rexiati
1

1.
Department of Urology, First Affiliated Hospital of Xinjiang Medical University, Urumchi, 830054, Xinjiang, China
Arch Med Sci 2020; 16 (5): 1207–1217
Online publish date: 2020/05/27
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Introduction
Bladder transitional cell carcinoma (BTCC) is one of the most prevalent human malignant diseases. Gemcitabine is commonly applied in the treatment of BTCC while acquired gemcitabine resistance has caused a severe impediment to recovery. This study aimed to investigate the function of DRAM2 in regulating gemcitabine resistance of BTCC.

Material and methods
GSE77883 was introduced to screen out the differentially expressed autophagy-related genes in T24 cells and gemcitabine-resistant T24-GEM cells. After establishing T24-GEM cells ourselves, aberrant expression of DRAM2 was detected by qRT-PCR and Western blot. After stably manipulating the expression of DRAM2 in T24 and T24-GEM cells, the changes of cell biological functions under gemcitabine treatment were compared, including cell viability, apoptosis and autophagy, using colony formation, flow cytometry and electron microscopy respectively.

Results
DRAM2 was up-regulated in gemcitabine-resistant T24-GEM cells. Silencing of DRAM2 in T24-GEM cells inhibited the cell autophagy induced by treatment with gemcitabine and contributed to attenuated gemcitabine resistance. Also, overexpression of DRAM2 in T24 cells enhanced the autophagy, strengthened the chemoresistance and decreased the cell apoptosis rate under the treatment with gemcitabine.

Conclusions
Our data suggested that downregulation of DRAM2 rescued the sensitivity of T24-GEM cells to gemcitabine, providing an appropriate therapeutic target for BTCC treatment.

keywords:

bladder cancer, DRAM2, autophagy, gemcitabine

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