@Article{Watała2008,
journal="Archives of Medical Science",
issn="1734-1922",
volume="4",
number="3",
year="2008",
title="Clinical researchImpaired action of nitric oxide on blood platelets in premature newborns",
abstract="  Introduction:  The influence of nitric oxide (NO) on blood platelets in premature newborns remains largely unexplained.   Material and methods:  The expression of the activated GPIIb-IIIa complex and  P-selectin were monitored by flow cytometry in whole blood platelets originating from 21 premature, 19 full-term neonates and 27 adults. Platelets were incubated with either freshly generated NO, L-arginine or NO donors (SNAP, GSNO) prior to their activation with collagen or ADP. Results: Platelets from preterm neonates showed extremely reduced reactivity compared to normal full-term neonates and adults, when activated by ADP, collagen or thrombin (P<0.001 or less). NO reduced reactivity of neonatal platelets activated with collagen to a lower extent compared to adult donors (up to 28-39% in full-term and 6-15% in preterm newborns compared to 25-49% in adults); the tendency was not so regular for ADP. The observed reduction in platelet function remained in proportion to newborns\&#8217; gestational age. Moreover, platelets from preterm neonates agonized with ADP showed reduced vulnerability to the action of L-NAME, an inhibitor of NO synthase, and L-cystamine, an inhibitor of guanylate cyclase. The immature responses in platelets from neonates, and especially preterm infants, are specific for various platelet activators, NO sources or modulators of the NO-dependent pathway.    Conclusions:  In spite of immaturity and reduced reactivity of platelets from newborns, their NO-dependent inhibition may be efficient to some extent even in most immature babies.",
author="Watała, Cezary
and Chudzik, Anita
and Tchórzewski, Henryk
and Gwoździński, Krzysztof",
pages="315--323",
url="https://www.termedia.pl/Clinical-research-Impaired-action-of-nitric-oxide-on-blood-platelets-in-premature-newborns,19,11206,1,1.html"
}