@Article{Styczyński2009,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="13",
number="6",
year="2009",
title="Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature",
abstract="A haploidentical transplant can be defined as a transplant that uses marrow or stem cells from a relative who is only half matched in HLA. Recently, a new regimen using graft CD3/CD19 depletion with anti-CD3- and anti-CD19-coated microbeads on a CliniMACS device was developed. CD3/CD19 depleted grafts contain not only CD34+ stem cells but also CD34-negative progenitors, natural killer, dendritic and graft-facilitating cells, thereby enhancing engraftment. We report a case description of a child using CD3/CD19 depleted grafts in a haploidentical stem cell transplant setting and a review of the available literature. A 3-year old boy was treated for very early relapse of acute lymphoblastic leukaemia. The myelo-ablative therapy consisted of weight-adjusted intravenous busulfan, flu-darabine, cyclophosphamide, and thymoglobuline. The reduction of CD3 load was 99.9% (i.e. 3.64 log scale); however, due to current CD3 load in the graft (2.7 × 105 CD3+/kg), GVHD prophylaxis was done with cyclosporine A and methotrexate. The only complication after transplantation was CMV reactivation, treated with pre-emptive therapy. Bone marrow biopsy at day +29 revealed normal haematopoiesis and donor chimerism 95%. The patient remains in remission at day +300. We can conclude from our observation and literature review that HLA haploidentical peripheral blood stem cell transplantation with CD3/CD19 depletion may be an effective and safe therapy for children with relapsed leukaemia.",
author="Styczyński, Jan
and Rochnowska, Małgorzata
and Dębski, Robert
and Hulek, Elżbieta
and Krenska, Anna
and Górnicka, Hanna
and Wojtylak, Paweł
and Wysocki, Mariusz",
pages="292--296",
url="https://www.termedia.pl/Haploidentical-allogeneic-haematopoietic-cell-transplantation-using-CD3-CD19-depletion-and-myeloablative-conditioning-a-case-report-and-a-review-of-the-literature,3,13994,1,1.html"
}