@Article{Dabek2010,
journal="Archives of Medical Science",
issn="1734-1922",
volume="6",
number="5",
year="2010",
title="Clinical researchAltered transcriptional activity of gene encoding GAPDH in peripheral blood mononuclear cells from patients with cardiac syndrome X – an important part in pathology of microvascular angina?",
abstract="  Introduction  : Cardiac syndrome X (CSX) is characterized by anginal pain with ECG suggestive of ischaemia and normal coronary arteries at angiography. Pathology of CSX involves microvascular dysfunction and is possibly linked with metabolic syndrome. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. The GAPDH gene is a “housekeeping” gene and is used for normalization in quantitative gene expression assays. The aim of the study was to evaluate GAPDH gene expression in CSX.      Material and methods  : The study was performed in 35 CSX patients and 10 con­trol subjects. mRNA was extracted from peripheral blood mononuclears and the mRNA was assessed by QRT-PCR.      Results  : GAPDH gene expression was enhanced in CSX patients vs. controls (93022 ±23837 copies/μg vs. 1067 ±240 copies/μg respectively; p < 0.001). Moreover, transcriptional activity of the GAPDH gene was heterogeneous within the CSX group.      Conclusions  : GAPDH gene expression is markedly enhanced in CSX, which reflects carbohydrate metabolism disturbances and makes the GAPDH gene unsuitable as an endogenous control in patients with CSX.",
author="Dabek, Jozefa
and Wilczok, Jakub
and Kulach, Andrzej
and Gasior, Zbigniew",
pages="709--712",
doi="10.5114/aoms.2010.17085",
url="http://dx.doi.org/10.5114/aoms.2010.17085"
}