@Article{Langfort2012,
journal="Kardiochirurgia i Torakochirurgia Polska/Polish Journal of Thoracic and Cardiovascular Surgery",
issn="1731-5530",
volume="9",
number="2",
year="2012",
title="TORAKOCHIRURGIAThe importance of immunohistochemistry for differentiation of lung adenocarcinoma and squamous cell carcinoma in small samples and cytology specimens",
abstract=" Introduction : In many cases typing of advanced lung non-small cell carcinoma (NSCLC), especially differentiation between adenocarcinoma (ADC) and squamous cell carcinoma (SCC),   requires special stains, mainly immunohistochemistry (IHC).    Methods : 53 cases of NSCLC diagnosed on small biopsies and cell block samples were evaluated. The panel of IHC antibodies comprised markers characteristic for ADC (TTF-1, napsin A, CK7) and for SCC (p63, CK5/6, CK34ßE12) in order to determine their value in diagnosis of NSCLC. Besides IHC, mucicarmine stain was tested. Results were correlated with the final diagnosis obtained from surgical specimens.   Results : In 57% of cases the subtype of NSCLC was established only by H+E stain. Using mucin stain and immunohistochemistry the accuracy of diagnosis increased by 22% to reach 79%. P63 turned out to be the most specific antibody, whose expression was seen in the vast majority of SQCC and in   a squamous component of other types of NSCLC. CK5/6 exhibited similar specificity to p63 but was lower in sensitivity. TTF-1 and napsin A were the most useful antibodies for ADC and   a positive reaction was seen in about 60% of ADC. Both markers were highly specific but, like p63, less sensitive. Since CK7 and CK34 proved less specific, their value in typing of NSCLC was limited. Mucin stain seemed to be a valuable marker but not very sensitive, although it was very helpful in diagnosis   of TTF-1 negative ADC.   Conclusions : IHC allows one to make a more accurate diagnosis than H+E stain alone. The most effective panel of IHC that facilitates subclassification of NSCLC in small samples and cell blocks comprised TTF-1, p63 and mucicarmine stain.",
author="Langfort, Renata
and Szołkowska, Małgorzata
and Szczepulska-Wójcik, Ewa
and Qandil, Naim
and Maksymiuk, Beata
and Giedronowicz, Dorota
and Rudziński, Piotr
and M. Orłowski, Tadeusz",
pages="202--209",
url="https://www.termedia.pl/TORAKOCHIRURGIA-The-importance-of-immunohistochemistry-for-differentiation-of-lung-adenocarcinoma-and-squamous-cell-carcinoma-in-small-samples-and-cytology-specimens,40,18927,1,1.html"
}