@Article{Semczuk2002,
journal="Menopause Review/Przegląd Menopauzalny",
issn="1643-8876",
volume="1",
number="2",
year="2002",
title="The correlation between genomic DNA total methylation and codon 12 K-ras gene point mutations in primary endometrial cancers",
abstract=" Materials and methods:   We analyzed the methyldeoxycytosine (m 5 dC) and the deoxycytosine content of genomic DNAs isolated from 44 primary endometrial cancers and 25 normal endometrium samples using enzymatic digestion into nucleotides,  32 P postlabelling, two-dimensional thin-layer chromatography on cellulose plates and bioimaging.   Results:  The overall m 5 dC of the uterine cancer DNAs (3.43%\±0.47%) was significantly higher than that of the normal proliferative endometrium (2.94%\±0.4%, p=0.003) and lower than that of the secretory endometrium DNAs (3.75%\±0.47%, p=0.03). In addition, RFLP-PCR revealed K-ras gene point mutations in 25% (11 cases) of the cancers. The m 5 dC of neoplasms with mutant K-ras was lower than that of cancers bearing the wild-type gene (3.19%\±0.31% vs. 3.51%\±0.48%, p=0.047). Among all endometrial cancer DNAs six were found to be hypomethylated, eight were hypermethylated, whereas the remaining 30 had m 5 dC within range of normal endometrium. Lower level of DNA methylation seemed to be associated with diminished tumor invasiveness. K-ras gene alterations were absent in all hypermethylated cancer DNAs.   Conclusions:  Our results suggest that alterations in overall DNA methylation seem to be a result of neoplastic transformation and could therefore be used as a prognostic molecular marker of endometrial cancer. DNA hypomethylation may facilitate the mutability of the K-ras gene.",
author="Semczuk, Andrzej
and Keith, Gérard
and A. Jakowicki, Jerzy
and Postawski, Krzysztof
and Miturski, Roman
and Baranowski, Włodzimierz",
url="https://www.termedia.pl/The-correlation-between-genomic-DNA-total-methylation-and-codon-12-K-ras-gene-point-mutations-in-primary-endometrial-cancers,4,1987,1,1.html"
}