@Article{Kulig2002,
journal="Menopause Review/Przegląd Menopauzalny",
issn="1643-8876",
volume="1",
number="3",
year="2002",
title="The 5A/6A promoter polymorphism of matrix metalloproteinase 3 (MMP-3) gene in menopausal women with ovarian cancer",
abstract=" Background:  Matrix metalloproteinases (MMPs) comprise family proteolytic enzymes that degrade of extracellular matrix components and therefore play an important role in tumour cell invasion and cancer metastasis. Overexpression of matrix metalloproteinase 3 (MMP-3 or stromelysin 1) gene has been demonstrated in various types of cancers and high level of MMP-3 protein in tumour is a poor prognostic factor for patients. The insertion (6A)/deletion (5A) polymorphism (5A/6A polymorphism) located at the promoter of the MMP-3 gene may have functional significance in the regulation of its expression.   Materials and methods:   In the present work the distribution of genotypes and frequencies of alleles of the 5A/6A polymorphism in subjects with ovarian cancer were investigated. Paraffin embedded tumour tissues were obtained from 50 menopausal women with ovarian cancer. The 5A/6A polymorphism were determined by PCR amplification using the allele specific primers.   Results:  The distribution of the genotypes of the 5A/6A polymorphism in both control and patients did not differ significantly (p>0.05) from those predicted by the Hardy-Weinberg distribution. Additionally, there were no significant differences (p >0.05) in genotype distributions and allele frequencies between subgroups assigned to histological stage.   Conclusion:  The results suggest that the 5A/6A polymorphism of MMP-3 gene may not be linked with appearance and development of ovarian cancer but further research, conducted on larger population, are needed to clarify this point.",
author="Kulig, Andrzej
and Smolarz, Beata
and Kozłowska, Elżbieta
and Romanowicz-Makowska, Hanna
and Szyłło, Krzysztof
and Niewiadomski, Mariusz
and Rechberger, Tomasz",
pages="21--25",
url="https://www.termedia.pl/The-5A-6A-promoter-polymorphism-of-matrix-metalloproteinase-3-MMP-3-gene-in-menopausal-women-with-ovarian-cancer,4,1999,1,1.html"
}