@Article{Wojas-Krawczyk2013,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="17",
number="6",
year="2013",
title="The analysis of ALK gene rearrangement by fluorescence in situ hybridisation in non-small cel lung cancer patients",
abstract=" Introduction :  ALK  gene rearrangement is observed in a small subset (3–7%) of non-small cell lung cancer (NSCLC) patients. The efficacy of crizotinib was shown in lung cancer patients harbouring  ALK  rearrangement. Nowadays, the analysis of  ALK  gene rearrangement is added to molecular examination of predictive factors.   Aim of the study:  The frequency of  ALK  gene rearrangement as well as the type of its irregularity was analysed by fluorescence  in situ  hybridisation (FISH) in tissue samples from NSCLC patients.   Material and methods:  The  ALK  gene rearrangement was analysed in 71 samples including 53 histological and 18 cytological samples. The analysis could be performed in 56 cases (78.87%), significantly more frequently in histological than in cytological materials. The encountered problem with  ALK  rearrangement diagnosis resulted from the scarcity of tumour cells in cytological samples, high background fluorescence noises and fragmentation of cell nuclei.    Results:  The normal  ALK  copy number without gene rearrangement was observed in 26 (36.62%) patients  ALK  gene polysomy without gene rearrangement was observed in 25 (35.21%) samples while in 3 (4.23%) samples  ALK  gene amplification was found.  ALK  gene rearrangement was observed in 2 (2.82%) samples from males, while in the first case the rearrangement coexisted with  ALK  amplification. In the second case, signet-ring tumour cells were found during histopathological examination and this patient was successfully treated with crizotinib with partial remission lasting 16 months.   Conclusions:  FISH is a useful technique for  ALK  gene rearrangement analysis which allows us to specify the type of gene irregularities.  ALK  gene examination could be performed in histological as well as cytological (cellblocks) samples, but obtaining a reliable result in cytological samples depends on the cellularity of examined materials.",
author="Wojas-Krawczyk, Kamila
and Krawczyk, Paweł Adam
and Ramlau, Rodryg Adam
and Szumiło, Justyna
and Kozielski, Jerzy
and Kalinka-Warzocha, Ewa
and Bryl, Maciej
and Knopik-Dąbrowicz, Alina
and Spychalski, Łukasz
and Szczęsna, Aleksandra
and Rydzik, Ewelina
and Milanowski, Janusz",
pages="484--492",
doi="10.5114/wo.2013.38758",
url="http://dx.doi.org/10.5114/wo.2013.38758"
}