@Article{Hoffman2015,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="19",
number="2",
year="2015",
title="Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro",
abstract=" The aim of the study : To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.    Material and methods : The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.    Results : ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose- and time-correlated response within high arsenic concentrations.   Conclusions : Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.",
author="Hoffman, Ewelina A.
and Gizelska, Katarzyna
and Mirowski, Marek
and Mielicki, Wojciech",
pages="108--112",
doi="10.5114/wo.2014.41390",
url="http://dx.doi.org/10.5114/wo.2014.41390"
}