@Article{Chen2014,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="18",
number="3",
year="2014",
title="Over-expression of beclin-1 in gallbladder carcinoma and its relationship with prognosis",
abstract=" Aim of the study:  We determined the expression of beclin-1 in gallbladder carcinoma, analyzed the association of beclin-1 expression with clinicopathologic parameters and prognosis, and investigated the relationship between microvessel density and beclin-1 expression.   Material and methods : Tissue microarray blocks containing 118 primary gallbladder tumor sites were used. Clinical parameters and prognostic data were collected. Expression of beclin-1 in these specimens was analyzed by immunohistochemical studies and confirmed by Western blotting. Microvessel density was counted using immunohistochemical staining.    Results:  The overall rate of over-expression of beclin-1 was 68.1% (80 of 118). Over-expression of beclin-1 was significantly associated with TNM stage and liver metastasis. Over-expression of beclin-1 has no significant relationship with age, sex, lymphatic metastasis, or tumor differentiation. Based on the Kaplan-Meier method, over-expression of beclin-1 showed no significant difference for the overall survival rate in patients with gallbladder cancer. The multivariate Cox regression analysis demonstrated that beclin-1 was not an independent prognostic factor for gallbladder cancer.   Conclusions : Beclin-1 might play roles in the development of gallbladder cancer. However, beclin-1 may not be an effective prognostic factor for gallbladder cancer.",
author="Chen, Yigang
and Yan, Jun
and Yu, Song
and Wang, Xiaohu
and Zheng, Qi",
pages="171--176",
doi="10.5114/wo.2014.41395",
url="http://dx.doi.org/10.5114/wo.2014.41395"
}