@Article{Abdel-Salam2014,
journal="Central European Journal of\ Immunology",
issn="1426-3912",
volume="39",
number="2",
year="2014",
title="Clinical immunologyExpression of CD11b and CD18 on polymorphonuclear neutrophils stimulated with interleukin-2",
abstract="  Background:  Interleukin-2 (IL-2) is a lymphocyte-activating and growth-promoting factor, and has been widely studied on T-cells and NK-cells. However, the interaction of polymorphonuclear neutrophils (PMNs) with IL-2 is poorly studied and thus, this study aimed at defining IL-2 participation in the expression of CD11b and CD18 on PMNs.    Material and methods:  PMNs were isolated from heparinized whole blood of healthy donors. Purified cells were incubated with  IL-2 (10 ng/ml) for 24 hours at 37°C in a humidified incubator with 5% CO2. After 24 hours’ incubation, surface molecules (CD11b and CD18) were measured by flow cytometry.    Results : Interestingly, the antibodies of IL-2Rβ chain (CD122-FITC) were found in all observed cells. The induction of CD11b mean fluorescence intensity (MFI) in highly purified PMNs stimulated with IL-2 was clearly increased recording 43% in comparison to the freshly isolated PMNs and the un-stimulated PMNs which were found to be 23% and 28% of CD11b, respectively. Furthermore, flow cytometry analysis demonstrated that the highly purified PMNs exposed to IL-2 showed an increase in CD18 MFI, recording 47% with respect to that of the freshly isolated PMNs and PMNs cultured with the medium alone which showed a small amount of 38% and 27%, respectively.    Conclusions : Results demonstrated that CD11b and CD18 had been acquired on the surface of the IL-2-   in vitro   -activated PMNs. These findings indicated that IL-2 may play a crucial role in PMNs migration. ",
author="Abdel-Salam, Bahaa K.A.
and Ebaid, Hossam",
pages="209--215",
doi="10.5114/ceji.2014.43725",
url="http://dx.doi.org/10.5114/ceji.2014.43725"
}