@Article{Radad2014,
journal="Folia Neuropathologica",
issn="1641-4640",
volume="52",
number="2",
year="2014",
title="Original articleNeuroprotective effect of rotigotine against complex I inhibitors, MPP+ and rotenone, in primary mesencephalic cell culture",
abstract="  Introduction:  Dopamine agonists are suggested to be more efficacious in treating Parkinson’s disease (PD) as they have neuroprotective properties in addition to their receptor-related actions.    Aim of the study : The present study was designed to investigate the neuroprotective effects of rotigotine, a D3/D2/D1 dopamine receptor agonist, against the two powerful complex I inhibitors, 1-methyl-4-phenylpyridinium (MPP + ) and rotenone, in primary mesencephalic cell culture relevant to PD.    Material and methods : Primary mesencephalic cell cultures were prepared from embryonic mouse mesencephala at gestation day 14. Three sets of cultures were treated with rotigotine alone, rotigotine and MPP + , and rotigotine and rotenone to investigate the effect of rotigotine on the survival of dopaminergic neurons against age-, MPP + - and rotenone-induced cell death. At the end of each treatment, cultures were fixed and stained immunohistochemically against tyrosine hydroxylase (TH). The effect of rotigotine against rotenone-induced reactive oxygen species (ROS) production was measured using CH-H2DCFDA fluorescence dye.    Results:  Rotigotine alone did not influence the survival of tyrosine hydroxylase immunoreactive (THir) neurons except at 10 µM, it significantly decreased the number of THir neurons by 40% compared to untreated controls. Treatment of cultures with 0.01 µM rotigotine rescued 10% of THir neurons against MPP + -induced cell death. Rotigotine was also found to significantly rescue 20% of THir neurons at 0.01 µM of rotenone-treated cultures. Using of CH-H2DCFDA fluorescence dye, it was found that rotigotine significantly attenuated ROS production compared to rotenone-treated cultures.    Conclusions : Rotigotine provides minor protection against MPP +  and rescues a significant number of THir neurons against rotenone in primary mesencephalic cell cultures relevant to PD.    ",
author="Radad, Khaled
and Scheller, Dieter
and Rausch, Wolf-Dieter
and Reichmann, Heinz
and Gille, Gabrielle",
pages="179--186",
doi="10.5114/fn.2014.43789",
url="http://dx.doi.org/10.5114/fn.2014.43789"
}