@Article{Shi2015,
journal="Central European Journal of\ Immunology",
issn="1426-3912",
volume="40",
number="3",
year="2015",
title="The p38 MAPK inhibitor SB203580 differentially modulates LPS-induced interleukin 6 expression in macrophages",
abstract=" The p38 mitogen-activated protein kinase (MAPK) plays a key role in lipopolysaccharide (LPS)-induced signal transduction pathways that lead to inflammatory cytokine synthesis in macrophages; however, whether the inhibition of p38 MAPK regulates LPS-induced inflammatory cytokine expression in different types of macrophages remains the subject of debate. Herein, we assessed whether the inhibition of p38 MAPK by SB203580 regulates LPS-induced expression of the inflammatory cytokines tumor necrosis factor  (TNF-) and interleukin 6 (IL-6) in RAW264.7 and resident peritoneal macrophages. Lipopolysaccharide stimulation of RAW264.7 macrophages or mouse resident peritoneal macrophages significantly increased TNF- and IL-6 production. The addition of SB203580 to cultures dramatically blocked LPS-induced TNF- production in RAW264.7 and mouse resident peritoneal macrophages, and dramatically blocked LPS-induced IL-6 production in RAW264.7 macrophages, but not in mouse resident peritoneal macrophages. Additionally, high concentrations of SB203580 resulted in increased IL-6 production. However, LPS-stimulation significantly up-regulated the mRNA transcript levels of TNF- and IL-6 in RAW264.7 and mouse resident peritoneal macrophages, whereas pretreatment with SB203580 dramatically down-regulated LPS-induced mRNA transcript levels of TNF- and IL-6 in these cells. Our data show that SB203580 differentially modulates LPS-induced production of the inflammatory cytokine IL-6 in two different sources of macrophages, and that this course of regulation occurs at the IL-6 mRNA post-transcriptional stage. ",
author="Shi, Qinghai
and Cheng, Liping
and Liu, Zhengxiang
and Hu, Keyan
and Ran, Jihua
and Ge, Di
and Fu, Jianfeng",
pages="276--282",
doi="10.5114/ceji.2015.54586",
url="http://dx.doi.org/10.5114/ceji.2015.54586"
}