@Article{Alomar2016,
journal="Polish Journal of Pathology",
issn="1233-9687",
volume="67",
number="2",
year="2016",
title="β-Catenin accumulation and S33F mutation of CTNNB1 gene in colorectal cancer in Saudi Arabia",
abstract="Several risk factors associated with colorectal cancer (CRC) have been identified including  β-catenin / CTNNB1  hotspot mutations. The levels of  β-catenin  within a cell are regulated via phosphorylation of the N terminus of  β-catenin  by GSK-3β. Thus far three serines (S33, 37, 45) and one threonine (T41) are considered to be the substrates for GSK-3β phosphorylation. In the present investigation an attempt was made to study the role of  β-catenin  mutations in exon-3 in 60 colorectal cancer patients from Kingdom of Saudi Arabia (KSA). The hot spot mutation region of  β-catenin  exon 3 was evaluated in matched tumor and normal tissues using PCR and direct sequencing. Sequencing of exon 3 of the  CTNNB1  gene revealed an activating mutation (S33F) in one of the tumor samples as compared to the normal tissue from the same patient where there was no such mutation found. Immunohistochemical staining showed the accumulation of  β-catenin  protein both in cytoplasm and in the nuclei of cancer cells as compared to normal tissue.",
author="Alomar, Suliman Y.
and Mansour, Lamjed
and Abuderman, Abdulwahab
and Alkhuriji, Afrah
and Arafah, Maha
and Alwasel, Saleh
and Harrath, Abdel Halim
and Almutairi, Mikhlid
and Trayhyrn, Paul
and Dar, Javid Ahmad",
pages="156--162",
doi="10.5114/pjp.2016.61452",
url="http://dx.doi.org/10.5114/pjp.2016.61452"
}